AB7204

Anti-NFkB Inducing Kinase NIK抗体

Name: NFkB Inducing Kinase NIK抗体 (ab7204)| Abcam中文官网
Brand: Abcam Limited
SKU: AB7204
Price: 4937 CNY
Availability: InStock
Rating: 4 (2 reviews)

Anti-NFkB Inducing Kinase NIK antibody

(2 Reviews)

(14 Publications)

Rabbit Polyclonal NFkB Inducing Kinase NIK antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K14 aa 750 to C-terminus.

查看别名

NIK, MAP3K14, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, Serine/threonine-protein kinase NIK, HsNIK

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (AB7204)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (AB7204)

ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-NFkB Inducing Kinase NIK antibody (AB7204)

Western blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.

All lanes:

Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)

Lane 1:

macrophages lysate

Lane 2:

NIK transfected cell lysate

Predicted band size: 104 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human MAP3K14 aa 750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99558

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Phosphorylates CHUK/IKKA, thereby promoting proteolytic processing of NFKB2/P100, which leads to NF-kappa-B activation via the non-canonical pathway (PubMed : 25406581, PubMed : 29230214). Has an essential role in the non-canonical NF-kappa-B signaling that regulates genes encoding molecules involved in B-cell survival, lymphoid organogenesis, and immune response (PubMed : 25406581). Could act in a receptor-selective manner.

See full target information MAP3K14

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1179827 PubMed37138866

2023

LCL161 enhances expansion and survival of engineered anti-tumor T cells but is restricted by death signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Arya Afsahi,Christopher M Silvestri,Allyson E Moore,Carly F Graham,Kaylyn Bacchiochi,Martine St-Jean,Christopher L Baker,Robert G Korneluk,Shawn T Beug,Eric C LaCasse,Jonathan L Bramson

Brazilian journal of medical and biological resear 53:e9271 PubMed32520202

2020

lncRNA PCGEM1 strengthens anti-inflammatory and lung protective effects of montelukast sodium in children with cough-variant asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxing Xu,Lingling Meng,Yuejuan Xie,Wei Guo

Nature communications 10:2247 PubMed31113953

2019

ZFYVE21 is a complement-induced Rab5 effector that activates non-canonical NF-κB via phosphoinosotide remodeling of endosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Caodi Fang,Thomas D Manes,Lufang Liu,Kevin Liu,Lingfeng Qin,Guangxin Li,Zuzana Tobiasova,Nancy C Kirkiles-Smith,Manal Patel,Jonathan Merola,Whitney Fu,Rebecca Liu,Catherine Xie,Gregory T Tietjen,Peter A Nigrovic,George Tellides,Jordan S Pober,Dan Jane-Wit

Oncology letters 15:4137-4142 PubMed29541178

2018

Tunicamycin suppresses breast cancer cell growth and metastasis via regulation of the protein kinase B/nuclear factor-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Wang,Wei Xiong,Yiyin Tang

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39:1010428317705574 PubMed28631556

2017

Kruppel-like factor 4 inhibits non-small cell lung cancer cell growth and aggressiveness by stimulating transforming growth factor-β1-meidated ERK/JNK/NF-κB signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Renzhi Yu,Lei Han,Xin Ni,Minghuan Wang,Ping Xue,Li Zhang,Mei Yuan

Current biology : CB 26:3288-3302 PubMed27889261

2016

NIK/MAP3K14 Regulates Mitochondrial Dynamics and Trafficking to Promote Cell Invasion.

Applications

ICC/IF

Species

Human

Ji-Ung Jung,Sowndharya Ravi,Dong W Lee,Kassandra McFadden,Michael L Kamradt,L Gerard Toussaint,Raquel Sitcheran

Oncogenesis 5:e231 PubMed27270613

2016

NIK regulates MT1-MMP activity and promotes glioma cell invasion independently of the canonical NF-κB pathway.

Applications

IHC

Species

Unspecified reactive species

C L Duran,D W Lee,J-U Jung,S Ravi,C B Pogue,L G Toussaint,K J Bayless,R Sitcheran

Nature cell biology 17:1327-38 PubMed26389665

2015

Non-canonical NF-κB signalling and ETS1/2 cooperatively drive C250T mutant TERT promoter activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Li,Qi-Ling Zhou,Wenjie Sun,Prashant Chandrasekharan,Hui Shan Cheng,Zhe Ying,Manikandan Lakshmanan,Anandhkumar Raju,Daniel G Tenen,Shi-Yuan Cheng,Kai-Hsiang Chuang,Jun Li,Shyam Prabhakar,Mengfeng Li,Vinay Tergaonkar

Reproductive sciences (Thousand Oaks, Calif.) 22:1618-26 PubMed26079730

2015

Knockdown of Eag1 Expression by RNA Interference Increases Chemosensitivity to Cisplatin in Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Hui,Zhang Lan,Lin Yue-li,Hong Li-lin,Huang Li-lin

Cell death and differentiation 22:2020-33 PubMed26045047

2015

NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

L Boutaffala,M J M Bertrand,C Remouchamps,G Seleznik,F Reisinger,M Janas,C Bénézech,M T Fernandes,S Marchetti,F Mair,C Ganeff,A Hupalowska,J-E Ricci,B Becher,J Piette,P Knolle,J Caamano,P Vandenabeele,M Heikenwalder,E Dejardin

View all publications

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Anti-NFkB Inducing Kinase NIK抗体

Anti-NFkB Inducing Kinase NIK antibody

Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (AB7204)

Western blot - Anti-NFkB Inducing Kinase NIK antibody (AB7204)