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AB264332

Anti-NFIB / NF1B2抗体

Anti-NFIB / NF1B2 antibody

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Rabbit Polyclonal NFIB / NF1B2 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NFIB aa 300-400.

查看别名

Nuclear factor 1 B-type, NF1-B, Nuclear factor 1/B, CCAAT-box-binding transcription factor, Nuclear factor I/B, TGGCA-binding protein, CTF, NF-I/B, NFI-B, NFIB

2 Images
Immunoprecipitation - Anti-NFIB / NF1B2 antibody (AB264332)
  • IP

Supplier Data

Immunoprecipitation - Anti-NFIB / NF1B2 antibody (AB264332)

NFIB / NF1B2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264332 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264332 at 1 μg/ml.

Lane 1 : ab264332 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in Hela whole cell lysate.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-NFIB / NF1B2 antibody (ab264332)

Predicted band size: 47 kDa

false

Western blot - Anti-NFIB / NF1B2 antibody (AB264332)
  • WB

Supplier Data

Western blot - Anti-NFIB / NF1B2 antibody (AB264332)

All lanes:

Western blot - Anti-NFIB / NF1B2 antibody (ab264332) at 0.1 µg/mL

Lane 1:

HeLa human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 47 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human NFIB aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

O00712

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Transcriptional activator of GFAP, essential for proper brain development (PubMed : 30388402). Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
See full target information NFIB

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