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重组RabMAb

重组Anti-NFE2抗体[EPR20463] (ab223845)

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Western blot - Anti-NFE2 antibody [EPR20463] (ab223845)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845)
  • Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] (ab223845)
  • Anti-NFE2 antibody [EPR20463] (ab223845)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20463] to NFE2
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-NFE2抗体[EPR20463]
    参阅全部 NFE2 一抗

  • 描述

    兔单克隆抗体[EPR20463] to NFE2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: K562, HEL and TF-1 whole cell lysates. IHC-P: Human placenta and malignant lymphoma of bone marrow tissue. Flow Cyt (intra): HEL cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab223845于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/400.
WB
1/1000. Predicted molecular weight: 41 kDa.
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
Flow Cyt (Intra)
1/400.
WB
1/1000. Predicted molecular weight: 41 kDa.
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

  • 组织特异性

    Expressed in hematopoietic cells and also in colon and testis.

  • 序列相似性

    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.

  • 结构域

    The PXY motifs are required for binding WW domains. PXY1 is required to promote transactivation of beta-globin and for hyperacetylation of histone H3, but not for binding to the HS2 promoter site.

  • 翻译后修饰

    Phosphorylated on serine residues (By similarity). In undifferentiated erythrocytes, phosphorylated by MAPK8 which then leads to ubiquitination and protein degradation.
    Sumoylated. Sumoylation is required for translocation to nuclear bodies PODs, anchoring to the gene loci, and transactivation of the beta-globin gene.
    Ubiquitinated mainly by 'Lys63'-linked ubiquitin. Polyubiquitination with 'Lys63'-linked ubiquitin by ITCH retains NFE2 in the cytoplasm preventing its transactivation activity. In undifferentiated erythrocyte, ubiquitinated after MAPK8-mediatd phosphorylation leading to protein degradation.

  • 细胞定位

    Nucleus > PML body. Cytoplasm. The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH.
  • Information by UniProt

  • 数据库链接

    • 别名

      • erythroid-derived 2 45 kDa subunit antibody
      • Leucine zipper protein NF-E2 antibody
      • Leucine zipper protein NFE2 antibody
      • NF E2 antibody
      • nfe2 antibody
      • NFE2_HUMAN antibody
      • Nuclear factor (erythroid derived 2), 45kDa antibody
      • nuclear factor (erythroid-derived 2), 45kD antibody
      • Nuclear factor antibody
      • nuclear factor, erythroid 2 antibody
      • Nuclear factor, erythroid derived 2 45 kDa subunit antibody
      • p45 antibody
      • p45 NF-E2 antibody
      • p45 NFE2 antibody
      • Transcription factor NF-E2 45 kDa subunit antibody
      • Transcription factor NFE2 45 kDa subunit antibody
      see all

    图片

    • Western blot - Anti-NFE2 antibody [EPR20463] (ab223845)
      Western blot - Anti-NFE2 antibody [EPR20463] (ab223845)
      All lanes : Anti-NFE2 antibody [EPR20463] (ab223845) at 1/1000 dilution

      Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
      Lane 2 : HEL (human bone marrow erythroleukemia cell line) whole cell lysate
      Lane 3 : TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 41 kDa
      Observed band size: 41 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845)

      Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling NFE2 with ab223845 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in neutrophils (arrow) in human placenta (PMID: 8645254) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845)

      Immunohistochemical analysis of paraffin-embedded malignant lymphoma of bone marrow tissue labeling NFE2 with ab223845 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on a subset of cells of malignant lymphoma of bone marrow is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] (ab223845)
      Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] (ab223845)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEL (human bone marrow erythroleukemia) cell line labeling NFE2 with ab223845 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). 

      Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Anti-NFE2 antibody [EPR20463] (ab223845)
      Anti-NFE2 antibody [EPR20463] (ab223845)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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    ab223845 尚未被引用在任何文献中。

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