Anti-NFATC4 (phospho S168 + S170)抗体(ab111929)
Key features and details
- Rabbit polyclonal to NFATC4 (phospho S168 + S170)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NFATC4 (phospho S168 + S170)抗体
参阅全部 NFATC4 一抗 -
描述
兔多克隆抗体to NFATC4 (phospho S168 + S170) -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human Breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+) -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab111929 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111929于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons. -
组织特异性
Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus. -
序列相似性
Contains 1 IPT/TIG domain.
Contains 1 RHD (Rel-like) domain. -
结构域
Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors. -
翻译后修饰
Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.
Ubiquitinated, leading to its degradation by the proteasome and reduced transcriptional activity. Ubiquitination and reduction in transcriptional activity can be further facilitated through GSK3B-dependent phosphorylation. Polyubiquitin linkage is mainly through 'Lys-48'. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. - Information by UniProt
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数据库链接
- Entrez Gene: 4776 Human
- Entrez Gene: 73181 Mouse
- Omim: 602699 Human
- SwissProt: Q14934 Human
- SwissProt: Q8K120 Mouse
- Unigene: 77810 Human
- Unigene: 27908 Mouse
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别名
- cytoplasmic 4 antibody
- NF ATc4 antibody
- NF-AT3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFATC4 (phospho S168 + S170) antibody (ab111929)ab111929 at 1/50 dilution staining NFATC4 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab111929 尚未被引用在任何文献中。