Anti-NFATC4抗体(ab3447)
Key features and details
- Rabbit polyclonal to NFATC4
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NFATC4抗体
参阅全部 NFATC4 一抗 -
描述
兔多克隆抗体to NFATC4 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human NFATC4 aa 887-902.
Sequence:RDLSGFPAPPGEEPPA
(Peptide available asab4979) -
阳性对照
- WB: MCF7, Jurkat, Raji, HeLa cells; ICC/IF: U251, HeLa, A431 cell lines; IP: MCF7 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa).
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ICC/IF |
1/100.
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IP |
Use at an assay dependent concentration.
3 μg |
说明 |
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WB
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 99 kDa). |
ICC/IF
1/100. |
IP
Use at an assay dependent concentration. 3 μg |
靶标
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功能
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons. -
组织特异性
Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus. -
序列相似性
Contains 1 IPT/TIG domain.
Contains 1 RHD (Rel-like) domain. -
结构域
Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors. -
翻译后修饰
Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by MTOR, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.
Ubiquitinated, leading to its degradation by the proteasome and reduced transcriptional activity. Ubiquitination and reduction in transcriptional activity can be further facilitated through GSK3B-dependent phosphorylation. Polyubiquitin linkage is mainly through 'Lys-48'. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. - Information by UniProt
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数据库链接
- Entrez Gene: 4776 Human
- Omim: 602699 Human
- SwissProt: Q14934 Human
- Unigene: 77810 Human
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别名
- cytoplasmic 4 antibody
- NF ATc4 antibody
- NF-AT3 antibody
see all
图片
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All lanes : Anti-NFATC4 antibody (ab3447) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : Raji whole cell lysate
Lane 4 : Ramos whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : U2OS whole cell lysate
Lane 7 : HeLa whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-rabbit at 1/20000 dilution
Predicted band size: 99 kDa -
Immunocytochemistry/Immunofluorescence analysis of NFATC4 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control - right) or with ab3447 at a dilution of 1:100 overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. NFATC4 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunocytochemistry/Immunofluorescence analysis of NFATC4 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control - right) or with ab3447 at a dilution of 1:20 overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. NFATC4 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunocytochemistry/Immunofluorescence analysis of NFATC4 in U251 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incubated without (control - right) or with ab3447 at a dilution of 1:20 overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. NFATC4 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunoprecipitation of NFATC4 was performed on MCF7 cells. The antigen:antibody complex was formed by incubating 500µg whole cell lysate with 3µg of rabbit polyclonal antibody recognizing NFATC4 (ab3447) overnight on a rocking platform at 4°C. The immune-complex was captured on 50µl Protein A/G Agarose. Captured immune-complexes were washed and proteins eluted with 5X Reducing Sample Loading Dye . Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1%Tween for at least 1 hour. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody at a dilution of 1:20000 for at least one hour. Membranes were washed and chemiluminescent detection performed.
All lanes :
Lane 1 : MCF7 cell lysate
Lane 2 : Immunoprecipitate
Secondary
All lanes : HRP-conjugated Goat anti-rabbit at 1/20000 dilution -
Immunofluorescent analysis of NFATC4 using anti-NFATC4 polyclonal antibody ( ab3447) (shown in green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing NFATC4 ( ab3447) at a dilution of 1:100 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab3447 被引用在 5 文献中.
- Kohno M et al. Enhancing calmodulin binding to cardiac ryanodine receptor completely inhibits pressure-overload induced hypertrophic signaling. Commun Biol 3:714 (2020). PubMed: 33244105
- Dittmann K et al. New roles for nuclear EGFR in regulating the stability and translation of mRNAs associated with VEGF signaling. PLoS One 12:e0189087 (2017). PubMed: 29253018
- Balakrishnan A et al. Temporal Analysis of Gene Expression in the Murine Schwann Cell Lineage and the Acutely Injured Postnatal Nerve. PLoS One 11:e0153256 (2016). IHC . PubMed: 27058953
- Gómez-Sintes R & Lucas JJ NFAT/Fas signaling mediates the neuronal apoptosis and motor side effects of GSK-3 inhibition in a mouse model of lithium therapy. J Clin Invest 120:2432-45 (2010). WB, IHC-FoFr ; Mouse . PubMed: 20530871
- Zhao X et al. [Expression and significance of COX-2 and its transcription factors NFAT3 and c-Jun in non-small cell lung cancer]. Zhongguo Fei Ai Za Zhi 13:1035-40 (2010). PubMed: 21081043