Anti-NFAT5抗体(ab226308)
Key features and details
- Rabbit polyclonal to NFAT5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NFAT5抗体
参阅全部 NFAT5 一抗 -
描述
兔多克隆抗体to NFAT5 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human NFAT5 aa 1481-1531. The exact sequence is proprietary.
Database link: O94916 -
阳性对照
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226308 was affinity purified using an epitope specific to NFAT5 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226308于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/25000. Predicted molecular weight: 166 kDa.
|
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| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/10000 - 1/25000. Predicted molecular weight: 166 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Plays a role in the inducible expression of genes. Regulates hypertonicity-induced cellular accumulation of osmolytes. -
组织特异性
Highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes. Also expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. -
序列相似性
Contains 1 RHD (Rel-like) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10725 Human
- Omim: 604708 Human
- SwissProt: O94916 Human
- Unigene: 371987 Human
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别名
- Glutamine rich protein H65 antibody
- KIAA0827 antibody
- NF AT5 antibody
see all
图片
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Western blot - Anti-NFAT5 antibody (ab226308)All lanes : Anti-NFAT5 antibody (ab226308) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 166 kDa
Exposure time: 30 secondsCell lysates were prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-NFAT5 antibody (ab226308)NFAT5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226308 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226308 at 0.4 µg/ml.
Lane 1: ab226308 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab226308 被引用在 1 文献中.
- Jiang Y et al. Transcription factor NFAT5 contributes to the glycolytic phenotype rewiring and pancreatic cancer progression via transcription of PGK1. Cell Death Dis 10:948 (2019). PubMed: 31827081