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Immunology Adaptive Immunity T Cells Non-CD
使用敲除细胞株进行验证

Anti-NFAT2抗体[7A6] (ab2796)

  • Datasheet
  • SDS
Reviews (9)Q&A (8)References (34)

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Western blot - Anti-NFAT2 antibody [7A6] (ab2796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)
  • Flow Cytometry - Anti-NFAT2 antibody [7A6] (ab2796)

Key features and details

  • Mouse monoclonal [7A6] to NFAT2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-NFAT2抗体[7A6]
    参阅全部 NFAT2 一抗
  • 描述

    小鼠单克隆抗体[7A6] to NFAT2
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Hamster, Non human primates
  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: FFPE human Hodgkin's lymphoma tissue sections.
  • 常规说明

    This monoclonal antibody is manufactured by Abcam. If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find more information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    7A6
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFATS
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • NFATs
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators

相关产品

  • Alternative Versions

    • Anti-NFAT2 antibody [7A6] - BSA and Azide free (ab264530)
  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human NFAT2 protein (ab64307)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2796于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
Use at an assay dependent concentration.
IHC-P (3)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

说明
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.
  • 组织特异性

    Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.
  • 序列相似性

    Contains 1 RHD (Rel-like) domain.
  • 结构域

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
    The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
    Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.
  • 翻译后修饰

    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Target information above from: UniProt accession O95644 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 4772 Human
    • Entrez Gene: 18018 Mouse
    • Entrez Gene: 100361818 Rat
    • Omim: 600489 Human
    • SwissProt: O95644 Human
    • SwissProt: O88942 Mouse
    • Unigene: 534074 Human
    • Unigene: 701518 Human
    • Unigene: 329560 Mouse
    see all
  • 别名

    • cytoplasmic 1 antibody
    • MGC138448 antibody
    • NF ATc antibody
    • NF ATc1 antibody
    • NF-ATc antibody
    • NF-ATc1 antibody
    • NF-ATc1.2 antibody
    • NFAC1_HUMAN antibody
    • NFAT 2 antibody
    • NFAT transcription complex cytosolic component antibody
    • NFATC 1 antibody
    • NFATc antibody
    • NFATc1 antibody
    • Nuclear factor of activated T cells cytoplasmic 1 antibody
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1 antibody
    • Nuclear factor of activated T cells cytosolic component 1 antibody
    • nuclear factor of activated T-cells 'c' antibody
    • Nuclear factor of activated T-cells antibody
    see all

图片

  • Western blot - Anti-NFAT2 antibody [7A6] (ab2796)
    Western blot - Anti-NFAT2 antibody [7A6] (ab2796)
    All lanes : Anti-NFAT2 antibody [7A6] (ab2796) at 5 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : Wild-type HAP1 cell lysate
    Lane 3 : NFATC1 knockout HAP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Observed band size: 75,80,90 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-NFAT2 antibody [7A6] staining at 5 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab2796 was shown to bind specifically to NFAT2. A band was observed at 75/80/90 kDa in wild-type HAP1 cell lysates with no signal observed at this size in NFATC1 knockout cell line. To generate this image, wild-type and NFATC1 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)

    IHC image of NFAT2 staining in a section of formalin-fixed paraffin-embedded normal human Hodgkin's lymphoma* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab2796, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT2 antibody [7A6] (ab2796)

    ab2796 staining human normal tonsil tissue. Staining is localized to cytoplasm and nucleus.
    Left panel: with primary antibody at 1 µg/ml. Right panel: Isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped under DePeX.

    Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Flow Cytometry - Anti-NFAT2 antibody [7A6] (ab2796)
    Flow Cytometry - Anti-NFAT2 antibody [7A6] (ab2796)

    Overlay histogram showing Jurkat cells stained with ab2796 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2796, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

实验方案

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (34)

发表研究结果有使用 ab2796?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2796 被引用在 34 文献中.

  • Sharlo KA  et al. Plantar Mechanical Stimulation Maintains Slow Myosin Expression in Disused Rat Soleus Muscle via NO-Dependent Signaling. Int J Mol Sci 22:N/A (2021). PubMed: 33573052
  • Meng KP  et al. Mechanosensing through YAP controls T cell activation and metabolism. J Exp Med 217:N/A (2020). PubMed: 32484502
  • Chakraborty S  et al. A Stronger Transcription Regulatory Circuit of HIV-1C Drives the Rapid Establishment of Latency with Implications for the Direct Involvement of Tat. J Virol 94:N/A (2020). PubMed: 32669338
  • Mira-Pascual L  et al. A Sub-Clone of RAW264.7-Cells Form Osteoclast-Like Cells Capable of Bone Resorption Faster than Parental RAW264.7 through Increased De Novo Expression and Nuclear Translocation of NFATc1. Int J Mol Sci 21:N/A (2020). PubMed: 31947698
  • Li G  et al. SIRT7 activates quiescent hair follicle stem cells to ensure hair growth in mice. EMBO J 39:e104365 (2020). PubMed: 32696520
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 17 Abreviews or Q&A

Western blot abreview for Anti-NFAT2 antibody [7A6]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 17 2020

Immunohistochemistry (Frozen sections) abreview for Anti-NFAT2 antibody [7A6]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 11 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (palatine tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
palatine tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 26 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Hannes Kaddatz

Verified customer

提交于 Jan 02 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Liver)
Permeabilization
Yes - 0.1%Triton x-100 in PB
Specification
Liver
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 16 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Umair Latif

Verified customer

提交于 Jun 18 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-NFAT2 antibody [7A6] - ChIP Grade

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hu pluripotent stem cell derived epicardial like)
Permeabilization
Yes - saponin
Specification
Hu pluripotent stem cell derived epicardial like
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 06 2016

Question

Does this antibody bind to the phosphorylated (cytoplasmic) or unphosphorylated (nuclear) form of NFAT2?

Read More

Abcam community

Verified customer

Asked on Dec 05 2013

Answer

Anti-NFAT2 [7A6] antibody - ChIP Grade, ab2796 binds to the cytoplasmic form within amino acids 1-654.

Read More

Elisa Thomas

Abcam Scientific Support

回复于 Dec 05 2013

Question

Please can you let me know what the antibody concentration is for: Anti-NFAT2 antibody [7A6] - ChIP Grade (ab2796).
Many Thanks,

Read More

Abcam community

Verified customer

Asked on Sep 03 2012

Answer

Thank you for your enquiry.

I can confirm that ab2796 NFAT2 antibody [7A6] antibody is sold asascites fluid. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for ascites, concentration of antibody is known to very between 5 - 10 mg/ml.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

回复于 Sep 03 2012

Question

Inquiry: Kindly advise currently available lot of ab2796. Thanks in advance for your assistance and reply.

Read More

Abcam community

Verified customer

Asked on Jul 02 2012

Answer

Thank you for your enquiry.

The lot we currently have available is #1312558 (GR73411-4).

I hope this information helps.

Read More

Abcam Scientific Support

回复于 Jul 02 2012

1-10 of 17 Abreviews or Q&A

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