AB288774

重组Anti-NFAT1抗体[1/NFAT-1] - BSA and Azide free

Anti-NFAT1 antibody [1/NFAT-1] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal NFAT1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

NFAT1, NFATP, NFATC2, NF-ATc2, NFATc2, NFAT pre-existing subunit, T-cell transcription factor NFAT1, NF-ATp

1 Images

Lab

Western blot - Anti-NFAT1 antibody [1/NFAT-1] - BSA and Azide free (AB288774)

This data was developed using ab288768, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

Negative control : HeLa (PMID : 1078663 and 16407284). The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 7479966). This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-NFAT1 antibody [1/NFAT-1] (<a href='/products/primary-antibodies/nfat1-antibody-1-nfat-1-ab288768'>ab288768</a>) at 1/1000 dilution

Lane 1:

Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 10 µg

Lane 2:

Ramos (human Burkitts lymphoma B lymphocyte), whole cell lysate at 10 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 120 kDa,140 kDa,36 kDa

true

Exposure time: 15s

不同偶联物与剂型 (1)

Unconjugated

Anti-NFAT1 antibody [1/NFAT-1]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

1/NFAT-1

亚型

IgG1

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

ab288774 is the carrier-free version of ab288768

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NFAT1 also known as NFATc2 is a transcription factor involved in gene regulation with a molecular weight of approximately 100 kDa. It belongs to the NFAT (nuclear factor of activated T-cells) family and is expressed in a variety of tissues including the immune system and the nervous system. NFAT1 plays an essential role in activating specific gene expressions by translocating into the nucleus upon cell stimulation. Researchers frequently use NFAT molecular weight to confirm its presence in protein analyses such as NFAT western blot techniques.

Biological function summary

NFAT1 influences immune response and development. It acts as part of a large complex with other transcription factors to bind DNA and regulate transcription of cytokines and other critical immune molecules. In activated T-cells NFAT1 controls the expression of genes important for immune function including interleukins. In neuronal tissues it contributes to neural growth and survival highlighting its multifunctional nature in different cell types.

Pathways

NFAT1 integrates into the calcineurin signaling pathway and plays a significant role in T-cell activation. It acts downstream of the calcium signaling cascade which leads to dephosphorylation and activation by the phosphatase calcineurin. NFAT1 works closely with proteins like AP-1 and GATA to facilitate the transcription of immune-related genes. These interactions highlight NFAT1's importance in immune signaling pathways which modulate immune surveillance and homeostasis.

NFAT1 associates with autoimmune diseases and certain cancers. In autoimmune conditions such as rheumatoid arthritis aberrant NFAT1 function leads to excessive cytokine production exacerbating inflammation. The protein also links to cancer development particularly in lymphoid malignancies where dysregulated NFAT1 activity contributes to uncontrolled cell proliferation. It interacts with oncogenic kinases and other dysregulated proteins in these disorders influencing both their progression and possible therapeutic targeting.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF (PubMed : 15790681). Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway (PubMed : 21871017). Is involved in the negative regulation of chondrogenesis (PubMed : 35789258). Recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca(2+) signaling microdomains where store-operated Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses.

See full target information NFATC2

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-NFAT1抗体[1/NFAT-1] - BSA and Azide free