JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB131473

Anti-NF2 / Merlin (phospho S518)抗体

Anti-NF2 / Merlin (phospho S518) antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal NF2 / Merlin phospho S518 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NF2 phospho S518 conjugated to Keyhole Limpet Haemocyanin.

查看别名

SCH, NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin

2 Images
Western blot - Anti-NF2 / Merlin (phospho S518) antibody (AB131473)
  • WB

Unknown

Western blot - Anti-NF2 / Merlin (phospho S518) antibody (AB131473)

All lanes:

Western blot - Anti-NF2 / Merlin (phospho S518) antibody (ab131473) at 1/500 dilution

Lane 1:

HUVEC cell extract

Lane 2:

HUVEC cell extract treated with IFN alpha

Predicted band size: 70 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-NF2 / Merlin (phospho S518) antibody (AB131473)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NF2 / Merlin (phospho S518) antibody (AB131473)

Immunofluorescent analysis of methanol-fixed HeLa cells labelling NF2 / Merlin (phospho S518) with ab131473 at 1/100 dilution.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human NF2 phospho S518 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P35240

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
ab131473 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NF2 gene produces a protein known as Neurofibromin 2 often called Merlin. The NF2 protein or Merlin protein plays a mechanical role in the cell as a tumor suppressor by stabilizing cellular structure and regulating cell growth. Its molar mass is approximately 70 kDa. NF2 protein is found mainly in the nervous system particularly in Schwann cells which are important for nerve function.
Biological function summary

The NF2 protein functions to control cell proliferation differentiation and motility. It associates with the cytoskeleton acting as a bridge between cell surface proteins and the actin cytoskeleton. Merlin as part of the ERM (ezrin radixin moesin) family contributes to the assembly of protein complexes at the cell membrane. Through its interactions it plays a significant role in maintaining proper cell signaling and structural integrity.

Pathways

NF2 protein integrates into the Hippo signaling pathway which regulates organ size by controlling cell growth and apoptosis. Within this pathway Merlin interacts with members such as YAP1 and LATS1/2 moderating their activity to prevent over-proliferation of cells. NF2 also connects with the mTOR pathway which is critical for cell metabolism and growth further emphasizing its regulatory functions in cellular homeostasis.

Mutations or deletions in the NF2 gene are associated with neurofibromatosis type 2 a condition that causes benign tumors on nerve tissues known as schwannomas. NF2 protein is linked to other proteins such as angiogenesis factors contributing to the development of these tumors. Schwannomatosis another disorder related to NF2 involves nerve sheath tumor formation with Merlin mutation playing a role indicating its importance in nervous system health.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
See full target information NF2 phospho S518
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
style
right-column

关联产品

Select an associated product type
Alternative Product
Primary Antibodies

AB2478

Anti-NF2 / Merlin (phospho S518) antibody

primary-antibodies

nf2-merlin-phospho-s518-antibody-ab2478

4

(1 reviews)

Alternative Product
Primary Antibodies

AB217016

Anti-NF2 / Merlin antibody

primary-antibodies

nf2-merlin-antibody-ab217016

0

(0 reviews)

Alternative Product
Primary Antibodies

AB84550

Anti-NF2 / Merlin antibody

primary-antibodies

nf2-merlin-antibody-ab84550

0

(0 reviews)

Alternative Product
Primary Antibodies

AB109244

Anti-NF2 / Merlin antibody [EPR2573(2)]

primary-antibodies

nf2-merlin-antibody-epr25732-ab109244

0

(0 reviews)

Alternative Product
Primary Antibodies

AB47378

Anti-NF2 / Merlin (phospho S518) antibody

primary-antibodies

nf2-merlin-phospho-s518-antibody-ab47378

2

(3 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com