AB76302

重组Anti-NF-kB p65 (phospho S536)抗体[EP2294Y]

Name: NF-kB p65(磷酸化S536)抗体[EP2294Y] (ab76302)| Abcam中文官网
Brand: Abcam Limited
SKU: AB76302
Price: 1960 CNY
Availability: InStock
Rating: 4 (7 reviews)

Anti-NF-kB p65 (phospho S536) antibody [EP2294Y]

20ul selling size
RabMAb
Recombinant
了解详情

(7 Reviews)

(485 Publications)

Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) is a rabbit monoclonal antibody detecting NF-kB p65 in Western Blot, IP, Dot Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 240 publications
- Trusted since 2009

查看别名

NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

4 Images

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Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)

ab76302 at 1/30 immunoprecipitating NF-kB p65 (phospho S536) in Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.

Lane 1 (input) : Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha (10μg)

Lane 2 (+) : ab76302 + Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76302 in Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.

For western blotting, ab76302 at 1/500 dilution (0.95 μg/ml) and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)

Predicted band size: 60 kDa

Observed band size: 65 kDa

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Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)

All lanes:

Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A and TNF-alpha whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 65 kDa

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Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)

All lanes:

Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

Lane 2:

C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate at 15 µg

Lane 3:

C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 65 kDa

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Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)

Dot blot analysis of INF- kB p65 (phospho S536) phospho peptide (Lane 1) and NF- kB p65 non-phospho peptide (Lane 2) labeling NF-kB p65 (phospho S536) with ab76302 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

不同偶联物与剂型 (1)

Carrier free

Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP2294Y

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

Dot, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Stimulation may be required to allow detection of the phosphorylated protein.
We recommend using NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 30 minutes as a positive control.

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反应性数据

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产品详情

Product Specifications
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, IP, WB in human, mouse, rat samples.
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) specifically detects NF-kB p65 Phospho-S536 (UniProt ID: Q04206; Molecular weight: 60kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) has been cited over 247 times in peer reviewed journals and is trusted by the scientific community.
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) has 7 independent reviews from customers.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed : 15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).

See full target information RELA phospho S536

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文献 (485)

Recent publications for all applications. Explore the full list and refine your search

Kidney diseases (Basel, Switzerland) 11:543-559 PubMed41064102

2025

Dihydroquercetin/Taxifolin Inhibits METTL3/mA/PIM2 Expression by Suppressing RIPK2 Phosphorylation Mediated NF-κB p65 Signaling Pathway to Alleviates Renal Ischemia-Reperfusion Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Shen,Xiao Wang,Zhongda Li,Jinyang Zhuang,Keqin Zhang

Probiotics and antimicrobial proteins : PubMed41026405

2025

Lacticaseibacillus rhamnosus LGG Suppresses Osteoclastogenesis via TLR6/NF-κB Modulation and Attenuates Ovariectomy-Induced Bone Loss in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kangjun Xiong,Jianghua Li,Yaqing Liu,Yang Pan,Yiping Huang,Dongdong Zhan,Lutong Zhang,Ming Tang,Jing Li,Haohao Sun

Scientific reports 15:33159 PubMed41006814

2025

Pedunculoside alleviates lipopolysaccharide-induced acute lung injury/acute respiratory distress syndrome by inhibiting NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yang,Zhi Xu,Qi Li,Guansong Wang,Jiancheng Xu

Journal of orthopaedic surgery and research 20:835 PubMed40999516

2025

Neuregulin 4 inhibits synovial macrophage pro-inflammatory polarization via ErbB4/Stat5b/NF-κB signaling to alleviate osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Daoliang Jiang,Xiaoli Xu,Wenjing Yang,Mengjia Tang,Yuping Zhang,Lingfeng Shi,Jiongyu Hu

Theranostics 15:9221-9239 PubMed40963908

2025

Neutrophil extracellular traps induce endothelial damage and exacerbate vasospasm in traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jinchao Wang,Lei Li,Jianye Xu,Dilmurat Gheyret,Kaiji Li,Xu Zhang,Haoran Jia,Ye Tian,Xiao Liu,Shenghui Li,Guili Yang,Yalong Gao,Ruilong Peng,Huajie Liu,Bin Liu,Jianfeng Zhuang,Cong Wang,Xin Chen,Yafan Liu,Bo Chen,Chuan Huang,Yuhan Li,Xin Chen,Jianning Zhang,Shu Zhang

Integrative cancer therapies 24:15347354251375464 PubMed40958203

2025

Sennoside A Modulates the Ferroptosis and Immune Evasion of Oral Squamous Cell Carcinoma Cells Through Inhibiting the NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Huo,Jianhua Qi,Qixuan Ren

CNS neuroscience & therapeutics 31:e70606 PubMed40916543

2025

Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Yao,Bingkai Ren,Qianhui Zhou,Yang Bai,Zhen Feng

ACS omega 10:36994-37003 PubMed40893218

2025

Potential Antiphotoaging Effect of Human Cathelicidin LL-37 Fragments and KR-12 Analogs on UVB-Induced HaCaT Cells and UVA-Induced HDF Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Menggeng Li,Jing Wang,Peng Shu,Xueqing Chen,Yizhen Yan,Jiangming Zhong,Nan Zhao,Ling Liang,Zhao Liu

Investigative ophthalmology & visual science 66:29 PubMed40793857

2025

Inhibiting The uPA/uPAR Pathway Affords Photoreceptor Resilience and Preserves Retinal Function in a Mouse Model of Retinitis Pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Rosario Amato,Alessio Canovai,Alberto Melecchi,Maria De Fenza,Linda Leone,Vincenzo Pavone,Daniele D'Alonzo,Maurizio Cammalleri,Massimo Dal Monte

Journal of experimental & clinical cancer research : CR 44:232 PubMed40781716

2025

Targeting NOTCH1-KEAP1 axis retards chronic liver injury and liver cancer progression via regulating stabilization of NRF2.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiao Chang,Meng Wang,Jia Li,Sihao Qi,Xiaojuan Yu,Jun Xu,Shengbin Shi

View all publications

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重组Anti-NF-kB p65 (phospho S536)抗体[EP2294Y]