AB218533

重组Anti-NF-kB p65 (acetyl K310)抗体[EPR21781] - ChIP Grade

Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade

20ul selling size
RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal NF-kB p65 acetyl K310 antibody. Suitable for ChIP, Dot, WB and reacts with Human, Synthetic peptide, Transfected cell lysate - Human samples. Cited in 5 publications.

查看别名

NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

5 Images

ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533)

ChIP

Supplier Data

ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533)

Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with and without 20 ng/ml TNF-α (ab9642) for 60 minutes according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 5 μg of ab218533 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach).

The ChIP data are consistent with the literature (PMID : 16135789).

Supplier Data

Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533)

Blocking/Dilution buffer : 5% NFDM/TBST.

We used a high sensitivity ECL substrate to develop this blot.

All lanes:

Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533) at 1/1000 dilution

All lanes:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 60 kDa

Observed band size: 65 kDa

false

Exposure time: 3min

Collaborator

Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533)

The p65 band runs higher in this blot because the protein contains a myc-tag.

Lanes 1 - 2:

Coomassie stain

Lanes 3 - 4:

Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533) at 1/1000 dilution

Lanes 1 and 3:

Acetylated p65 protein at 2 µg

Lanes 2 and 4:

K310R mutant protein at 2 µg

Secondary

All lanes:

IRDye® 800CW Goat anti-Rabbit IgG (H + L) at 1/15000 dilution

Predicted band size: 60 kDa

Observed band size: 75 kDa

false

This image is courtesy of an anonymous collaborator.

Supplier Data

Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533)

Blocking/Dilution buffer : 5% NFDM/TBST.

NF-κB p65 (acetyl K310) expression is induced by TNF-α and p300 acetyltransferases (PMID : 20160011, PMID : 12456660, PMID : 16135789).

All lanes:

Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533) at 1/2000 dilution

Lane 1:

HEK-293 transfected with NF-kB p65 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293 transfected with NF-kB p65 and p300 (aa1287-1663) expression vectors containing a myc-His-tag®, then treated with 20 ng/ml TNF-alpha (<a href='/products/proteins-peptides/recombinant-human-tnf-alpha-protein-ab9642'>ab9642</a>) for 60 minutes, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 60 kDa

Observed band size: 70 kDa

false

Exposure time: 37s

Supplier Data

Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533)

Dot blot analysis of NF-kB p65 (acetyl K310) labeled with ab218533 at 1/1000 dilution.

Lane 1 : NF-κB p65 (acetyl K310) peptide.
Lane 2 : NF-κB p65 non-acetyl peptide.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 2 minutes.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21781

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, Dot, ChIP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Synthetic peptide": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NF-kB p65 also known as RelA is a significant component of the NF-kB protein complex. This complex usually involves a molecular weight for p65 of approximately 65 kDa. NF-kB p65 is mechanistically a transcription factor that regulates genes involved in inflammation cell survival and immune response. Expression of p65 is widely seen in various cell types including immune cells and epithelial cells suggesting its role in numerous physiological processes.

Biological function summary

NF-kB p65 acts as part of the larger NF-kB complex usually forming a heterodimer with other family members like p50. This complex translocates to the nucleus upon activation where it binds specific DNA sequences to regulate gene expression. The p65 subunit is essential for transactivating target genes involved in immune and inflammatory responses. Its regulation is important for proper cellular functioning especially in the maintenance of immune homeostasis and inflammatory response.

Pathways

NF-kB p65 participates in critical pathways like the NF-kB signaling and Toll-like receptor pathways. These pathways are fundamental for initiating immune responses and contribute to the regulation of apoptosis and cellular stress responses. In the context of these pathways the IKK complex plays a pivotal role in NF-kB activation leading to the phosphorylation and subsequent degradation of inhibitors that retain NF-kB p65 in the cytoplasm therefore allowing its movement to the nucleus.

NF-kB p65 is implicated in conditions such as cancer and autoimmune diseases. Its dysregulation can lead to persistent activation of inflammatory pathways contributing to tumorigenesis and chronic inflammation. In the context of these diseases proteins such as the p50 subunit and the IKK complex are often involved reflecting the importance of tight regulation of NF-kB signaling in preventing pathological conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed : 15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).

See full target information RELA acetyl K310

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40709382

2025

Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis.

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Chih-Chao Yang,Ya Yue,Yi-Ting Wang,John Y Chiang,Ben-Chung Cheng,Tsuen-Wei Hsu,Yi-Ling Chen,Yi-Chen Li,Hon-Kan Yip

Journal of extracellular vesicles 13:e12427 PubMed38545803

2024

The small extracellular vesicle-mediated intercellular transformation of CXCR1 to CXCR1 tumour cells promotes the progression of head and neck squamous cell carcinoma.

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Qiaoshi Xu,Aoming Cheng,Bo Li,Xutengyue Tian,Zhengxue Han,Zhien Feng

Journal of nanobiotechnology 21:280 PubMed37598147

2023

Targeting macrophage M1 polarization suppression through PCAF inhibition alleviates autoimmune arthritis via synergistic NF-κB and H3K9Ac blockade.

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Jinteng Li,Feng Ye,Xiaojun Xu,Peitao Xu,Peng Wang,Guan Zheng,Guiwen Ye,Wenhui Yu,Zepeng Su,Jiajie Lin,Yunshu Che,Zhidong Liu,Pei Feng,Qian Cao,Dateng Li,Zhongyu Xie,Yanfeng Wu,Huiyong Shen

International journal of molecular sciences 22: PubMed34073604

2021

Phenolic Profiles of Red Wine Relate to Vascular Endothelial Benefits Mediated by SIRT1 and SIRT6.

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Nunzia D'Onofrio,Elisa Martino,Giuseppina Chianese,Francesca Coppola,Luigi Picariello,Luigi Moio,Maria Luisa Balestrieri,Angelita Gambuti,Martino Forino

Cells, tissues, organs 209:110-119 PubMed32772027

2020

IRF9 Affects the TNF-Induced Phenotype of Rheumatoid-Arthritis Fibroblast-Like Synoviocytes via Regulation of the SIRT-1/NF-κB Signaling Pathway.

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Fan Jiang,Hong-Yi Zhou,Li-Fang Zhou,Wei Zeng,Li-Han Zhao

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