AB19870

Anti-NF-kB p65 (acetyl K310)抗体

Name: NF-kB p65 (acetyl K310)抗体 (ab19870)| Abcam中文官网
Brand: Abcam Limited
SKU: AB19870
Price: 4836 CNY
Availability: InStock
Rating: 5 (6 reviews)

Anti-NF-kB p65 (acetyl K310) antibody

(6 Reviews)

(151 Publications)

Rabbit Polyclonal NF-kB p65 acetyl K310 antibody. Suitable for IP, Dot, WB and reacts with Mouse, Rat, Synthetic peptide samples. Cited in 151 publications.

查看别名

NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

5 Images

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Immunoprecipitation - Anti-NF-kB p65 (acetyl K310) antibody (AB19870)

Western Blot with ab19870 after p65 Immunoprecipitation : rabbit polyclonal to NF-kB p65 (acetyl K310) (ab19870; 2.5μg/ml) in 1% non-fat milk TBS-T incubated for 3 hours at room temperature. Exposure time : 1 min normal ECL. Tested samples : nuclear extracts (180 μg) of immortalized p65-/- mouse cells, complemented with the empty vector (pRRL), wild-type p65 (Wt) and non-acetylatable K310 (K310R). The samples tested were treated with deacetylase inhibitors HDACi (TSA + Nicotinamide) and TNF-alpha. The samples were immunoprecipitated with 2μg of alpha-p65 and subsequently analysed by Western blot with Rabbit polyclonal to NF-kB p65 (acetyl K310) (ab19870). Predicted band size = 65kDa, Observed band size = 75kDa. The p65 band runs higher in this SDS-PAGE blot as it contains a myc-tag.

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Immunoprecipitation - Anti-NF-kB p65 (acetyl K310) antibody (ab19870)

Predicted band size: 60 kDa

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This image is courtesy of Christine Buerki and Karin Rothgiesser, University of Zurich, Switzerland

Characteriser7094120****

Western blot - Anti-NF-kB p65 (acetyl K310) antibody (AB19870)

Observed band size : 75kDa

The p65 band runs higher in this blot because the protein contains a myc-tag.

Lanes 1 - 2:

Coomassie stain

Lanes 3 - 4:

Western blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870) at 1/500 dilution

Lanes 1 and 3:

Acetylated p65 protein at 2 µg

Lanes 2 and 4:

K310R mutant protein at 2 µg

Secondary

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IRDye® 800CW Goat anti-Rabbit IgG (H + L) at 1/15000 dilution

Predicted band size: 60 kDa

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This image is courtesy of Prof. Michael Hottiger's Lab, University of Zurich

Unknown

Western blot - Anti-NF-kB p65 (acetyl K310) antibody (AB19870)

ab19870 recognizes Rabbit polyclonal to NF-kB p65 (acetyl K310) specifically at ~75kDa (indicated by the arrow) is this SDS-PAGE blot. The p65 band runs higher than 65kDa in this SDS-PAGE blot as it contains a myc-tag. We are sure that the band at ~75kDa is p65 since p65 specific antibodies detect the same band in IP and WB and there is no signal in the p65 knock-out cell line with ab19870. A number of additonal bands are recognized by ab19870 when tested with endogenous p65 from whole cell extracts, we do not know the identity of these bands.

Tested samples : nuclear extracts (75μg) of immortalized p65-/- mouse cells, complemented with the empty ve

All lanes:

Western blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870) at 2.5 µg/mL

Lane 1:

pRRL untreated at 75 µg

Lane 2:

pRRL HDACi at 75 µg

Lane 3:

pRRL HDACi + TNF at 75 µg

Lane 4:

Wt untreated at 75 µg

Lane 5:

Wt HDACi at 75 µg

Lane 6:

Wt HDACi + phorbol myristate acetate at 75 µg

Lane 7:

K310R untreated at 75 µg

Lane 8:

K310R HDACi at 75 µg

Lane 9:

K310R HDACi + phorbol myristate acetate at 75 µg

Predicted band size: 60 kDa

Observed band size: 75 kDa

true

Exposure time: 1hr

This image is courtesy of Christine Buerki and Karin Rothgiesser, University of Zurich, Switzerland

Ap876607****

Western blot - Anti-NF-kB p65 (acetyl K310) antibody (AB19870)

All lanes:

Western blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870) at 1 µg/mL

All lanes:

Lung (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 60 kDa

Observed band size: 15 kDa,72 kDa

false

Exposure time: 4min

Collaborator

Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody (AB19870)

Rabbit polyclonal to NF-kB p65 (acetyl K310) (ab19870; 2.5μg/ml) in 1% non-fat milk TBS-T incubated for 3h at room temperature. Exposure time : 75 min normal ECL. This Dot blot demonstrates that ab19870 recognized upto 10ng of purified peptide on a PVDF membrane.

This image is courtesy of Christine Buerki and Karin Rothgiesser, University of Zurich, Switzerland

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat

应用

WB, IP, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Collaborator data suggests that immunoprecipitation of this antibody prior to Western blotting is required to obtain the best results (see images)", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2.5 µg/mL", "WB-species-notes": "Collaborator data suggests that immunoprecipitation of this antibody prior to Western blotting is required to obtain the best results (see images)", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NF-kB p65 also known as RelA is a significant component of the NF-kB protein complex. This complex usually involves a molecular weight for p65 of approximately 65 kDa. NF-kB p65 is mechanistically a transcription factor that regulates genes involved in inflammation cell survival and immune response. Expression of p65 is widely seen in various cell types including immune cells and epithelial cells suggesting its role in numerous physiological processes.

Biological function summary

NF-kB p65 acts as part of the larger NF-kB complex usually forming a heterodimer with other family members like p50. This complex translocates to the nucleus upon activation where it binds specific DNA sequences to regulate gene expression. The p65 subunit is essential for transactivating target genes involved in immune and inflammatory responses. Its regulation is important for proper cellular functioning especially in the maintenance of immune homeostasis and inflammatory response.

Pathways

NF-kB p65 participates in critical pathways like the NF-kB signaling and Toll-like receptor pathways. These pathways are fundamental for initiating immune responses and contribute to the regulation of apoptosis and cellular stress responses. In the context of these pathways the IKK complex plays a pivotal role in NF-kB activation leading to the phosphorylation and subsequent degradation of inhibitors that retain NF-kB p65 in the cytoplasm therefore allowing its movement to the nucleus.

NF-kB p65 is implicated in conditions such as cancer and autoimmune diseases. Its dysregulation can lead to persistent activation of inflammatory pathways contributing to tumorigenesis and chronic inflammation. In the context of these diseases proteins such as the p50 subunit and the IKK complex are often involved reflecting the importance of tight regulation of NF-kB signaling in preventing pathological conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed : 15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).

See full target information RELA acetyl K310

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文献 (151)

Recent publications for all applications. Explore the full list and refine your search

Chinese medicine 20:142 PubMed40890747

2025

Sodium tanshinone IIA sulfonate alleviates osteoarthritis through targeting SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Mao Xu,Xulei Sun,Xiao Ma,Zixuan Qin,Xin Gao,Xinxin Jin,Hongzhi Sun

Cell communication and signaling : CCS 23:165 PubMed40176138

2025

c-Ski is a novel repressor of NF-κB through interaction with p65 and HDAC1 in U937 cells.

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Unspecified application

Species

Unspecified reactive species

Yan Peng,Ren-Ping Xiong,Bo Wang,Xing Chen,Ya-Lie Ning,Yan Zhao,Nan Yang,Jing Zhang,Chang-Hong Li,Yuan-Guo Zhou,Ping Li

Nature communications 16:1363 PubMed39905015

2025

Adoptive cell transfer of piezo-activated macrophage rescues immunosuppressed rodents from life-threating bacterial infections.

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Unspecified application

Species

Unspecified reactive species

Xiaoyi Liu,Wenxiu Xu,Junkun Feng,Ying Wang,Kai Li,Yi Chen,Wenjun Wang,Weiwei Zhao,Shaohua Ge,Jianhua Li

Journal of biochemical and molecular toxicology 38:e70035 PubMed39552449

2024

Paeonol Alleviates Subarachnoid Hemorrhage Injury in Rats Through Upregulation of SIRT1 and Inhibition of HMGB1/TLR4/MyD88/NF-κB Pathway.

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Unspecified application

Species

Unspecified reactive species

Zhao Liu,Jun Zhu,Enyu Pan,Lujun Pang,Xiwei Zhou,Yanjun Che

Molecules (Basel, Switzerland) 29: PubMed39519747

2024

Co-Mn Complex Oxide Nanoparticles as Potential Reactive Oxygen Species Scavenging Agents for Pulmonary Fibrosis Treatment.

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Unspecified application

Species

Unspecified reactive species

Wuhao Yang,Hui Yuan,Hao Sun,Ting Hu,Yaping Xu,Yan Qiu,Yuhang Li

Cell reports 43:114488 PubMed39002124

2024

Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer's progression.

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Unspecified application

Species

Unspecified reactive species

Juan F Codocedo,Claudia Mera-Reina,Peter Bor-Chian Lin,Paul B Fallen,Shweta S Puntambekar,Brad T Casali,Nur Jury-Garfe,Pablo Martinez,Cristian A Lasagna-Reeves,Gary E Landreth

Experimental and therapeutic medicine 28:330 PubMed38979021

2024

Prevention of supercritical carbon dioxide fluid extract from Linnén on cutaneous squamous cell carcinomas progression following UV irradiation in mice.

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Unspecified application

Species

Unspecified reactive species

Qi-Hong Luo,Hong-Juan Chen,Qing-Yuan Zhong,Hao-En He,Ying-Qi Huang,You-Chen Liu,Bin Lan,Yao-Qi Wen,Si-Liang Deng,Xian-Hua Du,Bao-Qin Lin,Ya-Xian Zhan

CNS neuroscience & therapeutics 30:e14524 PubMed38105511

2023

Valproic acid ameliorates cauda equina injury by suppressing HDAC2-mediated ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingjie Kong,Fudong Li,Kaiqiang Sun,Xiaofei Sun,Jun Ma

Acta neuropathologica communications 11:182 PubMed37974279

2023

Neuronal dysfunction caused by FUSR521G promotes ALS-associated phenotypes that are attenuated by NF-κB inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Mari Carmen Pelaez,Antoine Desmeules,Pauline A Gelon,Bastien Glasson,Laetitia Marcadet,Alicia Rodgers,Daniel Phaneuf,Silvia Pozzi,Paul A Dutchak,Jean-Pierre Julien,Chantelle F Sephton

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 27:533-540 PubMed37884285

2023

Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodan Ma,Zhixin Guo,Wenhua Zhao,Li Chen

View all publications

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