Anti-Neutrophil Bactericidal蛋白BP30抗体(ab196732)
Key features and details
- Rabbit polyclonal to Neutrophil Bactericidal Protein BP30
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-Neutrophil Bactericidal蛋白BP30抗体 -
描述
兔多克隆抗体to Neutrophil Bactericidal蛋白BP30 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human Neutrophil Bactericidal Protein BP30 (C terminal).
Database link: P17213 -
阳性对照
- Jurkat, HepG2, SK-Hep1, mouse liver and mouse thymus extracts; Mouse lung tissue; A549 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab196732于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 54 kDa.
|
|
IHC-P |
1/50 - 1/200.
|
|
ICC/IF |
1/50 - 1/200.
|
说明 |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 54 kDa. |
IHC-P
1/50 - 1/200. |
ICC/IF
1/50 - 1/200. |
靶标
-
功能
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. -
组织特异性
Restricted to cells of the myeloid series. -
序列相似性
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
结构域
The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N-and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested. -
细胞定位
Secreted. Cytoplasmic granule membrane. Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. - Information by UniProt
-
数据库链接
- Entrez Gene: 671 Human
- Entrez Gene: 329547 Mouse
- Omim: 109195 Human
- SwissProt: P17213 Human
- SwissProt: Q67E05 Mouse
- Unigene: 529019 Human
- Unigene: 260883 Mouse
-
别名
- Bactericidal permeability-increasing protein antibody
- Bactericidal/permeability increasing protein antibody
- BPI antibody
see all
图片
-
All lanes : Anti-Neutrophil Bactericidal Protein BP30 antibody (ab196732) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : HepG2 cell extract
Lane 3 : SK-Hep1 cell extract
Lane 4 : Mouse liver extract
Lane 5 : Mouse thymus extract
Predicted band size: 54 kDa -
Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labeling Neutrophil Bactericidal Protein BP30 with ab196732 at 1/200 dilution.
-
Immunofluorescent analysis of A549 cells labeling Neutrophil Bactericidal Protein BP30 with ab196732 at 1/50 dilution.
Blue: DAPI for nuclear staining.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab196732 尚未被引用在任何文献中。