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Immunology Innate Immunity Macrophage / Inflamm.

Anti-Neutrophil抗体[NIMP-R14] (ab2557)

  • Datasheet
  • SDS
Reviews (25)Q&A (29)References (201)

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Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)

    Key features and details

    • Rat monoclonal [NIMP-R14] to Neutrophil
    • Suitable for: IHC-Fr
    • Reacts with: Mouse
    • Isotype: IgG2b

    Conjugates logo Related conjugates and formulations

    PE

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    概述

    • 产品名称

      Anti-Neutrophil抗体[NIMP-R14]
      参阅全部 Neutrophil 一抗
    • 描述

      大鼠单克隆抗体[NIMP-R14] to Neutrophil
    • 宿主

      Rat
    • 经测试应用

      适用于: IHC-Frmore details
    • 种属反应性

      与反应: Mouse
    • 免疫原

      Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils

    • 表位

      The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.
    • 常规说明

      The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.02% Sodium azide
      Constituent: 0.1% BSA
    • Concentration information loading...
    • 纯度

      Protein G purified
    • Primary antibody说明

      The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
    • 克隆

      单克隆
    • 克隆编号

      NIMP-R14
    • 骨髓瘤

      210RCY3-Ag123
    • 同种型

      IgG2b
    • 轻链类型

      unknown
    • 研究领域

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Cardiovascular
      • Blood
      • Other

    相关产品

    • Alternative Versions

      • PE Anti-Neutrophil antibody [NIMP-R14] (ab125159)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2557于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-Fr (4)
    Use at an assay dependent concentration.

    Tested with acetone-fixed tissue.

    说明
    IHC-Fr
    Use at an assay dependent concentration.

    Tested with acetone-fixed tissue.

    靶标

    • 细胞定位

      Cell membrane; Lipid-anchor, GPI-anchor
    • 数据库链接

      • Entrez Gene: 110453 Mouse
      • 别名

        • Ly-6B antibody
        • Ly6b antibody
        • Lymphocyte antigen 6B antibody
        • Lymphocyte antigen 6B Short name=Ly-6B antibody

      图片

      • Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)
        Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)

        Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (201)

      发表研究结果有使用 ab2557?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2557 被引用在 201 文献中.

      • Eliseeva SI  et al. Changes in lung immune cell infiltrates after electric field treatment in mice. Sci Rep 11:1453 (2021). PubMed: 33446928
      • Molenaar B  et al. Single-cell transcriptomics following ischemic injury identifies a role for B2M in cardiac repair. Commun Biol 4:146 (2021). PubMed: 33514846
      • Miao AF  et al. Hypoxia-inducible factor prolyl hydroxylase inhibitor roxadustat (FG-4592) protects against renal ischemia/reperfusion injury by inhibiting inflammation. Ren Fail 43:803-810 (2021). PubMed: 33966598
      • Brunner SM  et al. Galanin receptor 3 attenuates inflammation and influences the gut microbiota in an experimental murine colitis model. Sci Rep 11:564 (2021). PubMed: 33436730
      • Hazlewood JE  et al. Injection site vaccinology of a recombinant vaccinia-based vector reveals diverse innate immune signatures. PLoS Pathog 17:e1009215 (2021). PubMed: 33439897
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 54 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Brain)
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 May 30 2022

      Immunocytochemistry abreview for Anti-Neutrophil antibody [NIMP-R14]

      Average
      Abreviews
      Abreviews
      Application
      Immunocytochemistry
      Sample
      Mouse Cell (Heart)
      Permeabilization
      No
      Specification
      Heart
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Ann Kurian

      Verified customer

      提交于 Apr 27 2021

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rat Tissue sections (Mouse Liver Tissue)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate, pH 6.0
      Permeabilization
      No
      Specification
      Mouse Liver Tissue
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Apr 23 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Brain)
      Antigen retrieval step
      None
      Permeabilization
      No
      Specification
      Brain
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Mar 26 2020

      Immunohistochemistry (Frozen sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Lung)
      Permeabilization
      No
      Specification
      Lung
      Blocking step
      BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Carl Hobbs

      Verified customer

      提交于 Jun 09 2019

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (brain tissue)
      Antigen retrieval step
      None
      Permeabilization
      No
      Specification
      brain tissue
      Blocking step
      Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      HERR DR. Markus Kipp

      Verified customer

      提交于 Sep 07 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (spleen)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0
      Permeabilization
      No
      Specification
      spleen
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
      Fixative
      10% NBF
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 May 10 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (lung)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TE pH9
      Permeabilization
      No
      Specification
      lung
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jul 18 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
      Permeabilization
      No
      Specification
      Kidney
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Wesley Konsavage

      Verified customer

      提交于 Dec 22 2016

      Immunohistochemistry (Frozen sections) abreview for Anti-Neutrophil antibody [NIMP-R14]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Skin)
      Permeabilization
      No
      Specification
      Skin
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Dr. Victoria Bridgeman

      Verified customer

      提交于 Jun 23 2016

      1-10 of 54 Abreviews or Q&A

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