Anti-Neutrophil抗体[NIMP-R14] (ab2557)
![Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)](https://www.abcam.cn/ps/products/2/ab2557/Images/ab2557-282294-anti-neutrophil-antibody-nimp-r14-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)")
Key features and details
- Rat monoclonal [NIMP-R14] to Neutrophil
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2b
Related conjugates and formulations
概述
-
产品名称
Anti-Neutrophil抗体[NIMP-R14]
参阅全部 Neutrophil 一抗 -
描述
大鼠单克隆抗体[NIMP-R14] to Neutrophil -
宿主
Rat -
经测试应用
适用于: IHC-Frmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Neutrophil. Purified BALB/c mouse neutrophils
-
表位
The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C. -
常规说明
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
纯度
Protein G purified -
Primary antibody说明
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. -
克隆
单克隆 -
克隆编号
NIMP-R14 -
骨髓瘤
210RCY3-Ag123 -
同种型
IgG2b -
轻链类型
unknown -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (4) |
Use at an assay dependent concentration.
Tested with acetone-fixed tissue. |
| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. Tested with acetone-fixed tissue. |
靶标
-
细胞定位
Cell membrane; Lipid-anchor, GPI-anchor -
数据库链接
- Entrez Gene: 110453 Mouse
-
别名
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
图片
-
Immunohistochemistry (Frozen sections) - Anti-Neutrophil antibody [NIMP-R14] (ab2557)
Frozen sections of mouse spleen. ab2557 was used in a concentration of 5 μg/ml.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (201)
ab2557 被引用在 201 文献中.
- Eliseeva SI et al. Changes in lung immune cell infiltrates after electric field treatment in mice. Sci Rep 11:1453 (2021). PubMed: 33446928
- Molenaar B et al. Single-cell transcriptomics following ischemic injury identifies a role for B2M in cardiac repair. Commun Biol 4:146 (2021). PubMed: 33514846
- Miao AF et al. Hypoxia-inducible factor prolyl hydroxylase inhibitor roxadustat (FG-4592) protects against renal ischemia/reperfusion injury by inhibiting inflammation. Ren Fail 43:803-810 (2021). PubMed: 33966598
- Brunner SM et al. Galanin receptor 3 attenuates inflammation and influences the gut microbiota in an experimental murine colitis model. Sci Rep 11:564 (2021). PubMed: 33436730
- Hazlewood JE et al. Injection site vaccinology of a recombinant vaccinia-based vector reveals diverse innate immune signatures. PLoS Pathog 17:e1009215 (2021). PubMed: 33439897