AB269277

重组Anti-Neuroserpin抗体[EPR23329-221]

Name: Neuroserpin抗体[EPR23329-221] (ab269277)| Abcam中文官网
Brand: Abcam Limited
SKU: AB269277
Price: 1887 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Neuroserpin antibody [EPR23329-221]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
了解详情

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal Neuroserpin antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

PI12, SERPINI1, Neuroserpin, Peptidase inhibitor 12, Serpin I1, PI-12

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (AB269277)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (AB269277)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Neuroserpin with ab269277 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Negative control : No staining on human liver (PMID : 9070919).

The section was incubated with ab269277 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (AB269277)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neuroserpin with ab269277 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebrum (PMID : 17040209). The section was incubated with ab269277 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (AB269277)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neuroserpin with ab269277 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebrum (PMID : 17040209). The section was incubated with ab269277 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroserpin antibody [EPR23329-221] (AB269277)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Neuroserpin with ab269277 at 1/500 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse pancreatic islet (PMID : 9407089). The section was incubated with ab269277 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Unknown

Western blot - Anti-Neuroserpin antibody [EPR23329-221] (AB269277)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Human skeletal muscle (PMID : 9070919).

Exposure time : 3 mins.

All lanes:

Western blot - Anti-Neuroserpin antibody [EPR23329-221] (ab269277) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

false

Unknown

Western blot - Anti-Neuroserpin antibody [EPR23329-221] (AB269277)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 114 secs.

All lanes:

Western blot - Anti-Neuroserpin antibody [EPR23329-221] (ab269277) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 46 kDa

false

Unknown

Western blot - Anti-Neuroserpin antibody [EPR23329-221] (AB269277)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 mins.

All lanes:

Western blot - Anti-Neuroserpin antibody [EPR23329-221] (ab269277) at 1/1000 dilution

All lanes:

Rat pancreas tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 46 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Neuroserpin antibody [EPR23329-221] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23329-221

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Neuroserpin also known by its alternate name Serpin I1 is a member of the serine protease inhibitor family. It weighs about 44 kDa. Neuroserpin expresses mainly in the nervous system particularly in the brain and spinal cord where it regulates proteolytic activity. It localizes to the endoplasmic reticulum and is secreted to the extracellular space influencing neural processes.

Biological function summary

Neuroserpin plays an important role in nervous system development and maintenance. It inhibits tissue plasminogen activator (tPA) a protease involved in breaking down blood clots and controls extracellular matrix modifications. Neuroserpin does not form part of a complex but interacts with other key proteins for its function. Its regulation of tPA influences synaptic plasticity and protection of neurons from excessive proteolysis which is essential in maintaining proper brain function.

Pathways

Neuroserpin's activity is significant in the plasminogen activation system and neurotrophic pathways. It influences the balance of proteolytic activities that affect cell migration and synaptic remodeling. Related proteins in these pathways include proteases like tPA and neurotrophins like brain-derived neurotrophic factor (BDNF) which support neuronal survival.

Mutations in neuroserpin are linked to familial encephalopathy with neuroserpin inclusion bodies (FENIB) and Alzheimer's disease. FENIB is caused by neuroserpin polymerization and accumulation leading to neurodegeneration. In Alzheimer's disease neuroserpin interaction with amyloid precursor protein pathways contributes to plaque formation and neuronal dysfunction. Neuroserpin's connection with defective tPA regulation underlies its association with these neurodegenerative conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin (PubMed : 11880376, PubMed : 19265707, PubMed : 19285087, PubMed : 26329378, PubMed : 9442076). May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator (Probable).

See full target information SERPINI1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Pediatric research 97:1199-1208 PubMed39085403

2024

Neuroserpin normalization by mesenchymal stem cell therapy after encephalopathy of prematurity in neonatal rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lan-Wan Wang,Chien-Wei Hsiung,Ching-Ping Chang,Mao-Tsun Lin,Shyi-Jou Chen

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Neuroserpin抗体[EPR23329-221]