重组Anti-Neuropeptide Y抗体[EPR21877] (ab221145)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21877] to Neuropeptide Y
- Suitable for: mIHC, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Neuropeptide Y抗体[EPR21877]
参阅全部 Neuropeptide Y 一抗 -
描述
兔单克隆抗体[EPR21877] to Neuropeptide Y -
宿主
Rabbit -
经测试应用
适用于: mIHC, IHC-P, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human pheochromocytoma; Mouse cerebrum and adrenal gland; Rat adrenal gland tissues. IHC-Fr: Rat and mouse cerebrum tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21877 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab221145于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
mIHC |
1/2000.
|
|
IHC-P |
1/2000.
1:2000 dilution recommended for human, mouse and rat species. Perform antigen retrieval with universal HIER antigen retrieval reagent (10X) (ab208572). |
|
IHC-Fr |
1/100 - 1/500.
|
说明 |
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mIHC
1/2000. |
IHC-P
1/2000. 1:2000 dilution recommended for human, mouse and rat species. Perform antigen retrieval with universal HIER antigen retrieval reagent (10X) (ab208572). |
IHC-Fr
1/100 - 1/500. |
靶标
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功能
NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. -
组织特异性
One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla. -
序列相似性
Belongs to the NPY family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4852 Human
- Entrez Gene: 109648 Mouse
- Entrez Gene: 24604 Rat
- Omim: 162640 Human
- SwissProt: P01303 Human
- SwissProt: P57774 Mouse
- SwissProt: P07808 Rat
- Unigene: 1832 Human
see all -
别名
- C-flanking peptide of NPY antibody
- CPON antibody
- Neuropeptide tyrosine antibody
see all
图片
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Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; gray; Opal™690) at 1:2000 (0.263 μg/ml) [Panel B], anti-CYP11A1 stained on adrenal cortex (ab272494; green; Opal™520) at 1:10000 (0.053 μg/ml) [Panel C], and anti-Neuropeptide Y stained on chromaffin cells (ab221145; red; Opal™570) at 1:2000 (0.279 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of ab274418, ab272494, and ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded human pheochromocytoma tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Cytoplasmic staining in human pheochromocytoma (PMID: 15623622) is obserevd. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retreival.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Neuropeptide Y with ab221145 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat cerebrum tisssue labeling Neuropeptide Y with ab221145 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining in hypothalamic paraventricular nucleus of rat cerebrum (PMID: 19357287) is observed. Counterstained with DAPI (Nuclear).
Perform heat mediated antigen retrieval with sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. -
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on mouse cerebrum (PMID: 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Neuropeptide Y with ab221145 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on chromaffin cells of mouse adrenal gland medulla (PMID: 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). -
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Cytoplasmic staining in chromaffin cells of rat adrenal gland medulla (PMID: 15623622) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab221145 尚未被引用在任何文献中。