AB250464

Anti-Neuroligin 1 + Neuroligin 4 抗体 [EPR13108] - BSA and Azide free

Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal NLGNY antibody. Carrier free. Suitable for WB and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.

查看别名

KIAA0951, NLGN4Y, Neuroligin Y

2 Images

Supplier Data

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] - BSA and Azide free (AB250464)

This data was developed using ab181251, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (<a href='/products/primary-antibodies/neuroligin-1-neuroligin-4-antibody-epr13108-ab181251'>ab181251</a>) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Hela lysate at 20 µg

Lane 3:

A549 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 92 kDa

false

Lab

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] - BSA and Azide free (AB250464)

This data was developed using ab181251, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (<a href='/products/primary-antibodies/neuroligin-1-neuroligin-4-antibody-epr13108-ab181251'>ab181251</a>) at 1/1000 dilution

Lane 1:

His-tagged human recombinant Neuroligin 1 protein at 10 ng

Lane 2:

His-tagged human recombinant Neuroligin 2 protein at 10 ng

Lane 3:

His-tagged human recombinant Neuroligin 3 protein at 10 ng

Lane 4:

His-tagged human recombinant Neuroligin 4 protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 120 kDa

false

Exposure time: 5s

不同偶联物与剂型 (1)

Unconjugated

Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13108

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The immunogen of this antibody shows 92% sequence homology with the human Neuroligin-1 (UniProt ID Q8N2Q7) and 77% with human Neuroligin-2 (UniProt ID Q8NFZ4) and Neuroligin-3 (UniProt ID Q9NZ94).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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产品详情

ab250464 is the carrier-free version of ab181251.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Neuroligin 1 and Neuroligin 4 are cell adhesion proteins that play an important role in the development and function of synaptic connections in the nervous system. These proteins are part of the neuroligin family with Neuroligin 1 sometimes referred to as NLGN1 and Neuroligin 4 as NLGN4. Neuroligin 1 has a molecular mass of approximately 110 kDa while Neuroligin 4 has a mass of about 100 kDa. These proteins are primarily expressed in neurons where they contribute to the formation and maintenance of synaptic junctions between neurons.

Biological function summary

Neuroligin 1 and Neuroligin 4 facilitate the formation and maturation of excitatory and inhibitory synapses in the brain. These proteins work as part of a complex with their binding partners neurexins which reside on the presynaptic membrane to bridge the synaptic cleft. This interaction helps stabilize synaptic contacts and promotes the balance of synaptic transmission. Their role in synaptic specialization highlights their significance in maintaining proper neuronal network communication.

Pathways

Neuroligin 1 and Neuroligin 4 are positioned within the synaptic signaling pathways notably in the involvement of synapse organization and plasticity. These pathways ensure that synaptic signals are correctly transmitted and received which is essential for learning and memory processes. Neuroligins interact closely with the SHANK proteins and PSD-95 which are important components of the postsynaptic density. This interaction contributes to the structural and functional integrity of synapses allowing for precise cellular communication.

Mutations in Neuroligin 1 and Neuroligin 4 have been linked to neural developmental disorders including autism spectrum disorders and X-linked mental retardation. Aberrant neuroligin function affects synaptic stability and connectivity contributing to the pathophysiology of these conditions. Studies have identified interactions with proteins like neurexins and SHANK3 to be significant highlighting the complexity of neuronal circuitry affected in these disorders. Understanding this connection is key for exploring therapeutic strategies targeting synaptic dysfunction.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members.

See full target information NLGN4Y

其他靶点

NLGN1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 2:100360 PubMed34467244

2021

Secreted retrovirus-like GAG-domain-containing protein PEG10 is regulated by UBE3A and is involved in Angelman syndrome pathophysiology.

Applications

Unspecified application

Species

Unspecified reactive species

Nikhil J Pandya,Congwei Wang,Veronica Costa,Paul Lopatta,Sonja Meier,F Isabella Zampeta,A Mattijs Punt,Edwin Mientjes,Philip Grossen,Tania Distler,Manuel Tzouros,Yasmina Martí,Balazs Banfai,Christoph Patsch,Soren Rasmussen,Marius Hoener,Marco Berrera,Thomas Kremer,Tom Dunkley,Martin Ebeling,Ben Distel,Ype Elgersma,Ravi Jagasia

View all publications

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Anti-Neuroligin 1 + Neuroligin 4 抗体 [EPR13108] - BSA and Azide free