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重组

重组Anti-Neurogranin抗体[Ng2] - BSA and Azide free (ab273464)

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Western blot - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
  • Immunoprecipitation - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
  • Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [Ng2] to Neurogranin - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Neurogranin抗体[Ng2] - BSA and Azide free
    参阅全部 Neurogranin 一抗

  • 描述

    小鼠单克隆抗体[Ng2] to Neurogranin - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: IP, IHC-P, WBmore details
    不适用于: ICC/IF or IHC-Fr

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is considered to be commercially sensitive.

  • 阳性对照

    • WB: Human brain tissue lysate; Mouse hippicampus tissue lysate; Rat hippocampus tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue. IP: Mouse hippocampus tissue lysate.

  • 常规说明

    ab273464 is the carrier-free version of ab273443.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273464于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa).
说明
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa).
应用说明
Is unsuitable for ICC/IF or IHC-Fr.

靶标

  • 功能

    Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium.

  • 组织特异性

    In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease.

  • 序列相似性

    Belongs to the neurogranin family.
    Contains 1 collagen-like domain.
    Contains 1 IQ domain.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Protein kinase C substrate RC3 antibody
    • Calmodulin binding protein antibody
    • Hng antibody
    • NEUG(55-78) antibody
    • NEUG_HUMAN antibody
    • Neurogranin (protein kinase C substrate) antibody
    • Ng antibody
    • NRGN antibody
    • Protein kinase C substrate antibody
    • RC3 antibody
    see all

图片

  • Western blot - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Western blot - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    All lanes : Anti-Neurogranin antibody [Ng2] (ab273443) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Mouse hippocampus tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat hippocampus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 8 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?



    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Negative control: Mouse spleen (PMID: 20665622).
    The molecular weight observed is consistent with what has been described in the literature ( PMID: 20875798).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 secs; Lanes 2-4: 3 secs.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

  • Immunoprecipitation - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Immunoprecipitation - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Neurogranin was immunoprecipitated from 0.35 mg mouse hippocampus tissue lysate with ab273443 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273443 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

    Lane 1: Mouse hippocampus tissue lysate 10µg.

    Lane 2: ab273443 IP in mouse hippocampus tissue lysate.

    Lane 3: Mouse monoclonal IgG2b instead of ab273443 in Mouse hippocampus tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 58 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

  • Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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