Anti-Neurogranin抗体(ab99269)
Key features and details
- Goat polyclonal to Neurogranin
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Neurogranin抗体
参阅全部 Neurogranin 一抗 -
描述
山羊多克隆抗体to Neurogranin -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog
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免疫原
Synthetic peptide:
C-KIKSGERGRKG
, corresponding to internal sequence amino acids 45-55 of Human Neurogranin (NP_006167.1). -
阳性对照
- Human brain cerebral cortex lysate, Human brain hippocampus lysate, Mouse brain lysate and Rat brain lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab99269 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa).
A 1 hour primary incubation is recommended for this product. An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies. |
| 说明 |
|---|
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IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 8 kDa). A 1 hour primary incubation is recommended for this product. An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies. |
靶标
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功能
Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium. -
组织特异性
In the cerebral cortex, found in the cell bodies of neurons in layers II-VI, and in apical and basal dendrites of pyramidal neurons. Is not found in the dendrites in patients with Alzheimer disease. -
序列相似性
Belongs to the neurogranin family.
Contains 1 collagen-like domain.
Contains 1 IQ domain. - Information by UniProt
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数据库链接
- Entrez Gene: 4900 Human
- Entrez Gene: 64011 Mouse
- Entrez Gene: 64356 Rat
- Omim: 602350 Human
- SwissProt: P35722 Cow
- SwissProt: Q92686 Human
- SwissProt: P60761 Mouse
- SwissProt: Q04940 Rat
see all -
别名
- Protein kinase C substrate RC3 antibody
- Calmodulin binding protein antibody
- Hng antibody
see all
图片
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Western blot - Anti-Neurogranin antibody (ab99269)All lanes : Anti-Neurogranin antibody (ab99269) at 2 µg/ml
Lane 1 : Human cerebellum lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 8 kDa -
Western blot - Anti-Neurogranin antibody (ab99269)Anti-Neurogranin antibody (ab99269) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 8 kDa
Primary incubation was 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody (ab99269)ab99269 (3.8µg/ml) staining of paraffin embedded Human Cortex shows cytoplasm staining in the neuronal cell bodies. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
数据表及文件
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Datasheet download
文献 (1)
ab99269 被引用在 1 文献中.
- Sun X et al. Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy. Brain Behav Immun 82:178-187 (2019). PubMed: 31437533
