Anti-Neurofilament heavy polypeptide抗体[RNF402] (ab3966)
![Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)](https://www.abcam.cn/ps/products/3/ab3966/Images/ab3966-8436-anti-neurofilament-heavy-polypeptide-antibody-rnf402-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)")
Key features and details
- Mouse monoclonal [RNF402] to Neurofilament heavy polypeptide
- Suitable for: IHC-Fr
- Reacts with: Rat
- Isotype: IgM
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Neurofilament heavy polypeptide抗体[RNF402]
参阅全部 Neurofilament heavy polypeptide 一抗 -
描述
小鼠单克隆抗体[RNF402] to Neurofilament heavy polypeptide -
宿主
Mouse -
特异性
Axonal Marker. This antibody reacts with the intermediate filament protein of central and periferal nerve cells. In Western blotting this antibody is reactive with the Neurofilament heavy polypeptide protein only. This antibody reacts with both the phosphorylated and non-phosphorylated isoforms. -
经测试应用
适用于: IHC-Frmore details -
种属反应性
与反应: Rat -
免疫原
Tissue, cells or virus corresponding to Cow Neurofilament heavy polypeptide. Neurofilament preparation of calf brain tissue.
Database link: P12036 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
RNF402 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (1) |
1/10 - 1/100.
|
| 说明 |
|---|
|
IHC-Fr
1/10 - 1/100. |
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
疾病相关
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 24587 Rat
- SwissProt: P16884 Rat
- Unigene: 108194 Rat
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别名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)
Immunohistochemistry on frozen section of rat brain labeling Neurofilament heavy polypeptide with ab3966.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab3966 被引用在 6 文献中.
- Kim J et al. Nerve Repair and Orthodromic and Antidromic Nerve Grafts: An Experimental Comparative Study in Rabbit. Biomed Res Int 2020:5046832 (2020). PubMed: 31998792
- Liu L et al. Effects of oxygen generating scaffolds on cell survival and functional recovery following acute spinal cord injury in rats. J Mater Sci Mater Med 31:115 (2020). PubMed: 33247423
- Han S et al. Human placenta-derived mesenchymal stem cells loaded on linear ordered collagen scaffold improves functional recovery after completely transected spinal cord injury in canine. Sci China Life Sci 61:2-13 (2018). PubMed: 28527111
- Xiao Z et al. One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients. Sci China Life Sci 59:647-55 (2016). PubMed: 27333785
- Han S et al. The collagen scaffold with collagen binding BDNF enhances functional recovery by facilitating peripheral nerve infiltrating and ingrowth in canine complete spinal cord transection. Spinal Cord 52:867-73 (2014). PubMed: 25311846
- Haro H et al. Experimental chemonucleolysis with recombinant human matrix metalloproteinase 7 in human herniated discs and dogs. Spine J N/A:N/A (2013). Human . PubMed: 24295797