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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Other

Anti-Neurofilament heavy polypeptide抗体[RNF402] (ab3966)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (6)

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Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)

    Key features and details

    • Mouse monoclonal [RNF402] to Neurofilament heavy polypeptide
    • Suitable for: IHC-Fr
    • Reacts with: Rat
    • Isotype: IgM

    选择批间可重复性更高的重组抗体

    Product image
    Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-Neurofilament heavy polypeptide抗体[RNF402]
      参阅全部 Neurofilament heavy polypeptide 一抗
    • 描述

      小鼠单克隆抗体[RNF402] to Neurofilament heavy polypeptide
    • 宿主

      Mouse
    • 特异性

      Axonal Marker. This antibody reacts with the intermediate filament protein of central and periferal nerve cells. In Western blotting this antibody is reactive with the Neurofilament heavy polypeptide protein only. This antibody reacts with both the phosphorylated and non-phosphorylated isoforms.
    • 经测试应用

      适用于: IHC-Frmore details
    • 种属反应性

      与反应: Rat
    • 免疫原

      Tissue, cells or virus corresponding to Cow Neurofilament heavy polypeptide. Neurofilament preparation of calf brain tissue.
      Database link: P12036

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      单克隆
    • 克隆编号

      RNF402
    • 骨髓瘤

      Sp2/0-Ag14
    • 同种型

      IgM
    • 研究领域

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Neuroscience
      • Cell Adhesion Proteins
      • Cytoskeletal Proteins
      • Intermediate Filaments
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Intermediate Filaments
      • Class IV
      • Neurofilaments
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Axon marker
      • Neuroscience
      • Development

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
      • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
      • Goat Anti-Mouse IgM H&L (ab9167)
      • Rabbit Anti-Mouse IgM mu chain (ab9175)
    • Recombinant Protein

      • Recombinant Human Neurofilament heavy polypeptide protein (ab158986)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3966于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-Fr (1)
    1/10 - 1/100.
    说明
    IHC-Fr
    1/10 - 1/100.

    靶标

    • 功能

      Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
    • 疾病相关

      Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
    • 序列相似性

      Belongs to the intermediate filament family.
    • 翻译后修饰

      There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
      Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
    • Target information above from: UniProt accession P12036 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 24587 Rat
      • SwissProt: P16884 Rat
      • Unigene: 108194 Rat
      • 别名

        • 200 kDa neurofilament protein antibody
        • CMT2CC antibody
        • Nefh antibody
        • Neurofilament heavy polypeptide 200kDa antibody
        • Neurofilament heavy polypeptide antibody
        • Neurofilament triplet H protein antibody
        • NF H antibody
        • NF-H antibody
        • NFH antibody
        • NFH_HUMAN antibody
        see all

      图片

      • Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)
        Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966)

        Immunohistochemistry on frozen section of rat brain labeling Neurofilament heavy polypeptide with ab3966.

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (6)

      发表研究结果有使用 ab3966?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3966 被引用在 6 文献中.

      • Kim J  et al. Nerve Repair and Orthodromic and Antidromic Nerve Grafts: An Experimental Comparative Study in Rabbit. Biomed Res Int 2020:5046832 (2020). PubMed: 31998792
      • Liu L  et al. Effects of oxygen generating scaffolds on cell survival and functional recovery following acute spinal cord injury in rats. J Mater Sci Mater Med 31:115 (2020). PubMed: 33247423
      • Han S  et al. Human placenta-derived mesenchymal stem cells loaded on linear ordered collagen scaffold improves functional recovery after completely transected spinal cord injury in canine. Sci China Life Sci 61:2-13 (2018). PubMed: 28527111
      • Xiao Z  et al. One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients. Sci China Life Sci 59:647-55 (2016). PubMed: 27333785
      • Han S  et al. The collagen scaffold with collagen binding BDNF enhances functional recovery by facilitating peripheral nerve infiltrating and ingrowth in canine complete spinal cord transection. Spinal Cord 52:867-73 (2014). PubMed: 25311846
      • Haro H  et al. Experimental chemonucleolysis with recombinant human matrix metalloproteinase 7 in human herniated discs and dogs. Spine J N/A:N/A (2013). Human . PubMed: 24295797

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-Neurofilament heavy polypeptide antibody [RNF402]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rabbit Cell (peripheral nerve tissue)
      Permeabilization
      Yes - Triton-X-100
      Specification
      peripheral nerve tissue
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Zuzana Koci

      Verified customer

      提交于 Aug 23 2021

      Question

      The isotype is IgM - what is the allotype?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 04 2005

      Answer

      Thank you for your enquiry. This antibody is IgM kappa, we don't have any additional information. If you have any additional questions, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 Nov 08 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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