AB207176

重组Anti-Neurofilament heavy polypeptide抗体[EPR20020]

Anti-Neurofilament heavy polypeptide antibody [EPR20020]

RabMAb
Recombinant
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(44 Publications)

Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) is a rabbit monoclonal antibody detecting Neurofilament heavy polypeptide in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

查看别名

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on Human cerebellum is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on the normal Human cerebrum tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control : No staining on human kidney.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebral cortex tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Positive staining on rat cerebral cortex is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling Neurofilament heavy polypeptide with ab207176 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on Neuro-2a cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Positive staining on mouse hippocampus is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on neurons of the rat cerebral cortex is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on mouse cerebellum is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 1 minute; Lane 2 : 30 seconds.

Lane 1:

Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/1000 dilution

Lane 2:

Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/2000 dilution

Lane 1:

Human midbrain lysate at 10 µg

Lane 2:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

Secondary

Lane 1:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 112 kDa

Observed band size: 200-210 kDa

false

Supplier Data

Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Blocking/Dilution buffer : 5% NFDM/TBST.

The MW observed is consistent with the literature : PMID : 25374587; PMID : 144556.

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/1000 dilution

All lanes:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 112 kDa

Observed band size: 200-210 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1/2 : 3 seconds; Lane 3/4 : 1 second.

The MW observed is consistent with the literature : PMID : 25374587; PMID : 144556.

Lanes 1 and 3:

Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/1000 dilution

Lanes 2 and 4:

Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/2000 dilution

Lane 1:

Mouse cerebellum lysate at 10 µg

Lane 2:

Mouse brain lysate at 10 µg

Lane 3:

Rat cerebellum lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 112 kDa

Observed band size: 200-210 kDa

false

不同偶联物与剂型 (5)

Carrier free

Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free
Carrier free

Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) - BSA and Azide free
Unconjugated

Anti-NEFH antibody [EPR20020] - Neuronal Marker - Chicken IgY (Chimeric)
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Neurofilament heavy polypeptide antibody [EPR20020]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Neurofilament heavy polypeptide antibody [EPR20020]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20020

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ICC/IF, IHC-P, WB, IHC-Fr

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Neurofilament heavy polypeptide?
Anti-Neurofilament heavy polypeptide [EPR20020] (ab207176) specifically detects a band for Neurofilament heavy polypeptide (UniProt: P12036) at a molecular weight of 112kDa.

Trusted by the scientific community
Anti-Neurofilament heavy polypeptide [EPR20020] (ab207176) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20020] also available for your convenience: ab207176, Carrier free - ab223139, ab317042, Carrier free - ab317043, Alexa Fluor® 647 - ab321784, Alexa Fluor® 488 - ab322308

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.

Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFH

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文献 (44)

Recent publications for all applications. Explore the full list and refine your search

European journal of histochemistry : EJH 69: PubMed40984791

2025

Expression of nestin, parvalbumin and otoferlin during cochlear development in the mouse: an immunofluorescence study.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Liu,Yongchun Zhang,Cheng Liang,Shujuan Yang

Regenerative therapy 30:641-655 PubMed40896181

2025

Printable biomaterials for 3D brain regenerative scaffolds: An in vivo biocompatibility assessment.

Applications

Unspecified application

Species

Unspecified reactive species

Maylis Combeau,Nina Colitti,Julien Clauzel,Franck Desmoulin,Adrien Brilhault,Juliette Fitremann,Mickaël Chabbert,Matthew L Becker,Sébastien Blanquer,Lorenne Robert,Melissa Parny,Isabelle Raymond-Letron,Carla Cirillo,Isabelle Loubinoux

Nature 646:462-473 PubMed40836096

2025

Cancer-induced nerve injury promotes resistance to anti-PD-1 therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Erez N Baruch,Frederico O Gleber-Netto,Priyadharsini Nagarajan,Xiayu Rao,Shamima Akhter,Tuany Eichwald,Tongxin Xie,Mohammad Balood,Adebayo Adewale,Shorook Naara,Hinduja N Sathishkumar,Shajedul Islam,William McCarthy,Brandi J Mattson,Renata Ferrarotto,Michael K Wong,Michael A Davies,Sonali Jindal,Sreyashi Basu,Karine Roversi,Amin Reza Nikpoor,Maryam Ahmadi,Ali Ahmadi,Catherine Harwood,Irene Leigh,Dennis Gong,Paulino Tallón de Lara,Derrick L Tao,Tara M Davidson,Nadim J Ajami,Andrew Futreal,Kunal Rai,Veena Kochat,Micah Castillo,Preethi Gunaratne,Ryan P Goepfert,Sharia D Hernandez,Nikhil I Khushalani,Jing Wang,Stephanie S Watowich,George A Calin,Michael R Migden,Mona Yuan,Naijiang Liu,Yi Ye,William L Hwang,Paola D Vermeer,Nisha J D'Silva,Yuri L Bunimovich,Dan Yaniv,Jared K Burks,Javier Gomez,Patrick M Dougherty,Kenneth Y Tsai,James P Allison,Padmanee Sharma,Jennifer A Wargo,Jeffrey N Myers,Sebastien Talbot,Neil D Gross,Moran Amit

Communications biology 8:1134 PubMed40739377

2025

Inhibition of inner ear macrophage phagocytosis alleviates cisplatin-induced ototoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Zhang,Weijian Zhang,Xiaotong Huang,Moyang Li,Haidi Yang

Journal of neuroinflammation 22:161 PubMed40544251

2025

Pro-repair macrophages driven by CGRP rescue white matter integrity following intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Huaping Huang,Yirui Kuang,Yang Chen,Yi Zhang,Jiayin Zhou,Xian Yu,Yonghe Zheng,Lingxin Cai,Wanglu Hu,Liansheng Gao,Haijian Wu,Hui Ling,Xiao Dong,Hang Zhou,Xiaobo Yu,Yucong Peng,Gao Chen,Xiaoyu Wang,Wei Yan

CNS neuroscience & therapeutics 31:e70431 PubMed40365715

2025

Hydrogen Sulfide Modulates Microglial Polarization and Remodels the Injury Microenvironment to Promote Functional Recovery After Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Xinyi Jia,Yuqi Zhang,Haibin Shi,Yuhui Sun,Yaobo Liu

The European journal of neuroscience 61:e70125 PubMed40329506

2025

Changes of the Primary Cilia in Alzheimer's Disease Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Angyang Guo,Han Wang,Yan Zhang,Hongbin Huang

iScience 28:111940 PubMed40034120

2025

Mrgprb4-lineage neurons indispensable in pressure induced pleasant sensation are polymodal.

Applications

Unspecified application

Species

Unspecified reactive species

Longhua Du,Hongyi Cheng,Xiang Cui,Qianan Cao,Xia Li,Shuya Wang,Xiaoxi Wang,Yun Liu,Bing Zhu,Xinyan Gao,Kun Liu

Journal of nanobiotechnology 23:152 PubMed40016738

2025

Pectin-Zein-IPA nanoparticles promote functional recovery and alleviate neuroinflammation after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xianghang Chen,Beini Wang,Abdullah Al Mamun,Kaiyi Du,Shengfu Wang,Qianqian Hu,Xinyuan Chen,Yang Lu,Anyu Du,Yueqi Wu,Jiaqin Shao,Shuangshuang Wang,Chang Jiang,Kailiang Zhou,Siwang Hu,Jian Xiao

Cell & bioscience 15:11 PubMed39881390

2025

Trehalose enhances macrophage autophagy to promote myelin debris clearance after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhida Ma,Congpeng Meng,Xiang Wang,Yuanzhe Zhao,Jingwen Wang,Yihao Chen,Yiteng Li,Yan Jiang,Fangru Ouyang,Jianjian Li,Meige Zheng,Li Cheng,Juehua Jing

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