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重组RabMAb

重组Anti-Neurofilament heavy polypeptide抗体[EPR20020-129] - BSA and Azide free (Capture) (ab274433)

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Sandwich ELISA - Anti-Neurofilament heavy polypeptide antibody [EPR20020-129] - BSA and Azide free (Capture) (ab274433)
  • Anti-Neurofilament heavy polypeptide antibody [EPR20020-129] - BSA and Azide free (Capture) (ab274433)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20020-129] to Neurofilament heavy polypeptide - BSA and Azide free (Capture)
  • Suitable for: Sandwich ELISA
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647

概述

  • 产品名称

    Anti-Neurofilament heavy polypeptide抗体[EPR20020-129] - BSA and Azide free (Capture)
    参阅全部 Neurofilament heavy polypeptide 一抗

  • 描述

    兔单克隆抗体[EPR20020-129] to Neurofilament heavy polypeptide - BSA and Azide free (Capture)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Sandwich ELISAmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    ab274433 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse NEFH. The recommended pair for sandwich ELISA is:
         Capture: ab274433, Mouse NEFH Capture Antibody (unconjugated)
         Detector: ab274434, Mouse NEFH Detector Antibody (unconjugated)
    The reference range value is 125 - 8000 pg/ml.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab274433于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR22806-69] to Neurofilament heavy polypeptide - BSA and Azide free (Detector) (ab274434).
说明
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR22806-69] to Neurofilament heavy polypeptide - BSA and Azide free (Detector) (ab274434).

靶标

  • 功能

    Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

  • 疾病相关

    Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.

  • 序列相似性

    Belongs to the intermediate filament family.

  • 翻译后修饰

    There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
    Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
    Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 200 kDa neurofilament protein antibody
      • CMT2CC antibody
      • Nefh antibody
      • Neurofilament heavy polypeptide 200kDa antibody
      • Neurofilament heavy polypeptide antibody
      • Neurofilament triplet H protein antibody
      • NF H antibody
      • NF-H antibody
      • NFH antibody
      • NFH_HUMAN antibody
      see all

    图片

    • Sandwich ELISA - Anti-Neurofilament heavy polypeptide antibody [EPR20020-129] - BSA and Azide free (Capture) (ab274433)
      Sandwich ELISA - Anti-Neurofilament heavy polypeptide antibody [EPR20020-129] - BSA and Azide free (Capture) (ab274433)
      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272479).
    • Anti-Neurofilament heavy polypeptide antibody [EPR20020-129] - BSA and Azide free (Capture) (ab274433)
      Anti-Neurofilament heavy polypeptide antibody [EPR20020-129] - BSA and Azide free (Capture) (ab274433)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab274433？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab274433 尚未被引用在任何文献中。

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