重组Anti-Neurofilament heavy polypeptide抗体[EP673Y] (ab40796)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)](https://www.abcam.cn/ps/products/40/ab40796/Images/ab40796-53989-anti-neurofilament-heavy-polypeptide-antibody-ep673y-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP673Y] to Neurofilament heavy polypeptide
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Neurofilament heavy polypeptide抗体[EP673Y]
参阅全部 Neurofilament heavy polypeptide 一抗 -
描述
兔单克隆抗体[EP673Y] to Neurofilament heavy polypeptide -
宿主
Rabbit -
特异性
ab40796 recognises Neuofilaments (NF). -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Neurofilament heavy polypeptide aa 650-750. The exact sequence is proprietary.
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阳性对照
- WB: Human, mouse and rat brain tissue lysate. IHC-P: Human gliocytoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP673Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40796于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/2000. Detects a band of approximately 200 kDa (predicted molecular weight: 112 kDa).
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| IHC-P |
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000. Detects a band of approximately 200 kDa (predicted molecular weight: 112 kDa). |
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IHC-P
1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
疾病相关
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 4744 Human
- Entrez Gene: 380684 Mouse
- Entrez Gene: 24587 Rat
- Omim: 162230 Human
- SwissProt: P12036 Human
- SwissProt: P19246 Mouse
- SwissProt: P16884 Rat
- Unigene: 198760 Human
see all -
别名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain glyoma tissue labelling
Neurofilament heavy polypeptide with unpurified ab40796.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)](https://www.abcam.cn/ps/products/40/ab40796/Images/ab40796-242123-anti-neurofilament-heavy-polypeptide-antibody-ep673y-western-blot.jpg)
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution (purified) + Human brain tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 200-220 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)](https://www.abcam.cn/ps/products/40/ab40796/Images/ab40796-242124-anti-neurofilament-heavy-polypeptide-antibody-ep673y-western-blot.jpg)
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)All lanes : Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 200-220 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)](https://www.abcam.cn/ps/products/40/ab40796/Images/ab40796-53990-anti-neurofilament-heavy-polypeptide-antibody-ep673y-western-blot.jpg)
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution (unpurified) + Rat brain tissue lysate
Predicted band size: 112 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)](https://www.abcam.cn/ps/products/40/ab40796/Images/ab40796-242125-anti-neurofilament-heavy-polypeptide-antibody-ep673y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling Neurofilament heavy polypeptide with purified ab40796 at 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)](https://www.abcam.cn/ps/products/40/ab40796/Images/ab40796-7-benefits-of-recombinant-antibodies.png)
Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab40796 被引用在 9 文献中.
- Huang Y et al. Hearing Protection Outcomes of Analog Electrode Arrays Coated with Different Drug-Eluting Polymer Films Implanted into Guinea Pig Cochleae. Drug Des Devel Ther 15:3443-3450 (2021). PubMed: 34413631
- Sperry ZJ et al. Spatial models of cell distribution in human lumbar dorsal root ganglia. J Comp Neurol 528:1644-1659 (2020). PubMed: 31872433
- Wu M et al. Wnt signaling contributes to withdrawal symptoms from opioid receptor activation induced by morphine exposure or chronic inflammation. Pain 161:532-544 (2020). PubMed: 31738230
- Liu AM et al. Human adipose tissue- and umbilical cord-derived stem cells: which is a better alternative to treat spinal cord injury? Neural Regen Res 15:2306-2317 (2020). PubMed: 32594054
- Mahdee A et al. Evidence for changing nerve growth factor signalling mechanisms during development, maturation and ageing in the rat molar pulp. Int Endod J 52:211-222 (2019). PubMed: 30099752
- Pinacho R et al. Altered CSNK1E, FABP4 and NEFH protein levels in the dorsolateral prefrontal cortex in schizophrenia. Schizophr Res 177:88-97 (2016). PubMed: 27236410
- Oh SS et al. Neurodegeneration and early lethality in superoxide dismutase 2-deficient mice: a comprehensive analysis of the central and peripheral nervous systems. Neuroscience 212:201-13 (2012). IHC-Fr ; Mouse . PubMed: 22516022
- Namavari A et al. In vivo serial imaging of regenerating corneal nerves after surgical transection in transgenic thy1-YFP mice. Invest Ophthalmol Vis Sci 52:8025-32 (2011). PubMed: 21896845
- Serrano-Pozo A et al. Beneficial effect of human anti-amyloid-beta active immunization on neurite morphology and tau pathology. Brain 133:1312-27 (2010). PubMed: 20360050