Anti-Neurofilament heavy polypeptide抗体(ab8135)
Key features and details
- Rabbit polyclonal to Neurofilament heavy polypeptide
- Suitable for: IHC-FrFl, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Neurofilament heavy polypeptide抗体
参阅全部 Neurofilament heavy polypeptide 一抗 -
描述
兔多克隆抗体to Neurofilament heavy polypeptide -
宿主
Rabbit -
特异性
The antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa). -
经测试应用
适用于: IHC-FrFl, WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide. From bovine spinal cord.
Database link: F1MSQ6 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.03% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
疾病相关
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 380684 Mouse
- Entrez Gene: 24587 Rat
- SwissProt: P19246 Mouse
- SwissProt: P16884 Rat
- Unigene: 298283 Mouse
- Unigene: 108194 Rat
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别名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
图片
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Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)
Immunohistological analysis of a mouse hippocampus section stained with ab8135 at a dilution 1:2,000 in red, and co-stained with a mouse mAb to myelin basic protein (MBP) at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.
Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.
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Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)All lanes : Anti-Neurofilament heavy polypeptide antibody (ab8135) at 1/10000 dilution
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat spinal cord tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : Mouse spinal cord tissue lysate
数据表及文件
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SDS download
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Datasheet download
文献 (137)
ab8135 被引用在 137 文献中.
- Kodati B et al. Early-Onset Glaucoma in egl1 Mice Homozygous for Pitx2 Mutation. Biomedicines 10:N/A (2022). PubMed: 35327318
- Gibson JM et al. Perinatal methadone exposure attenuates myelination and induces oligodendrocyte apoptosis in neonatal rat brain. Exp Biol Med (Maywood) 247:1067-1079 (2022). PubMed: 35475383
- Kulesskaya N et al. Low-Molecular Weight Protamine Overcomes Chondroitin Sulfate Inhibition of Neural Regeneration. Front Cell Dev Biol 10:865275 (2022). PubMed: 35547817
- Scalise S et al. Human iPSC Modeling of Genetic Febrile Seizure Reveals Aberrant Molecular and Physiological Features Underlying an Impaired Neuronal Activity. Biomedicines 10:N/A (2022). PubMed: 35625812
- Yin G et al. Upregulated lncARAT in Schwann cells promotes axonal regeneration by recruiting and activating proregenerative macrophages. Mol Med 28:76 (2022). PubMed: 35768768