Anti-Neurofilament heavy polypeptide抗体(ab4680)
Key features and details
- Chicken polyclonal to Neurofilament heavy polypeptide
- Suitable for: ICC, IHC-FrFl, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgY
概述
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产品名称
Anti-Neurofilament heavy polypeptide抗体
参阅全部 Neurofilament heavy polypeptide 一抗 -
描述
鸡多克隆抗体to Neurofilament heavy polypeptide -
宿主
Chicken -
经测试应用
适用于: ICC, IHC-FrFl, WBmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Preservative: 0.032% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
IgY fraction -
纯化说明
This is an IgY prep, similar to a mammalian ammonium sulphate IgG prep from serum. It is virtually pure IgY and we estimate that about 5% of material is specific to the immunogen. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Isotype control
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Recombinant Protein
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | (1) |
Use at an assay dependent concentration.
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| IHC-FrFl | (1) |
Use at an assay dependent concentration.
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| WB |
1/1e+006. Predicted molecular weight: 200-220 kDa.
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| 说明 |
|---|
|
ICC
Use at an assay dependent concentration. |
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IHC-FrFl
Use at an assay dependent concentration. |
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WB
1/1e+006. Predicted molecular weight: 200-220 kDa. |
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
疾病相关
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 528842 Cow
- Entrez Gene: 4744 Human
- Entrez Gene: 380684 Mouse
- Entrez Gene: 24587 Rat
- Omim: 162230 Human
- SwissProt: P12036 Human
- SwissProt: P19246 Mouse
- SwissProt: P16884 Rat
see all -
别名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
图片
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Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Immunohistological analysis of a rat cerebellum section stained with ab4680 at a dilution 1:5,000 in red, and co-stained with rabbit pAb to GFAP at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.
Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free floating sections were stained.
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Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)All lanes : Anti-Neurofilament heavy polypeptide antibody (ab4680) at 1/20000 dilution
Lane 1 : Rat spinal cord lysate
Lane 2 : Mouse spinal cord lysate
Lane 3 : Cow spinal cord lysate
Predicted band size: 200-220 kDa -
Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Mixed neuron/glial cultures stained with a mouse monoclonal antibody to neurofilament subunit NF-L (green) and ab4680, chicken antibody to neurofilament heavy polypeptide. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.
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Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (ab4680)Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section). -
Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (ab4680)Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section).
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Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)Image from Wirt SE et al, J Cell Biol. 2010 Nov 15;191(4):809-25. Epub 2010 Nov 8, Fig 4. DOI 10.1083/jcb.201003048ab4680 staining Neurofilament heavy polypeptide (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.
数据表及文件
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SDS download
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Datasheet download
文献 (96)
ab4680 被引用在 96 文献中.
- Hosoya M et al. The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development. FEBS J 288:325-353 (2021). PubMed: 32323465
- Ewan EE et al. Ascending dorsal column sensory neurons respond to spinal cord injury and downregulate genes related to lipid metabolism. Sci Rep 11:374 (2021). PubMed: 33431991
- Jiang Y et al. Basic fibroblast growth factor accelerates myelin debris clearance through activating autophagy to facilitate early peripheral nerve regeneration. J Cell Mol Med 25:2596-2608 (2021). PubMed: 33512767
- Triplet EM et al. The thrombin receptor links brain derived neurotrophic factor to neuron cholesterol production, resiliency and repair after spinal cord injury. Neurobiol Dis 152:105294 (2021). PubMed: 33549720
- Andreassi C et al. Cytoplasmic cleavage of IMPA1 3' UTR is necessary for maintaining axon integrity. Cell Rep 34:108778 (2021). PubMed: 33626357