Anti-Neurofilament heavy polypeptide抗体(ab134459)
Key features and details
- Chicken polyclonal to Neurofilament heavy polypeptide
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgY
概述
-
产品名称
Anti-Neurofilament heavy polypeptide抗体
参阅全部 Neurofilament heavy polypeptide 一抗 -
描述
鸡多克隆抗体to Neurofilament heavy polypeptide -
宿主
Chicken -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse -
免疫原
Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide.
-
常规说明
Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
-
纯度
IgY fraction -
纯化说明
Immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in buffer, and then filter-sterilized. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134459于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells.
|
说明 |
---|
ICC/IF
1/2000 - 1/5000. Use 2% paraformaldehyde-fixed cells. |
靶标
-
功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
疾病相关
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
-
数据库链接
- Entrez Gene: 380684 Mouse
- SwissProt: P19246 Mouse
- Unigene: 298283 Mouse
-
别名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (ab134459)Picture courtesy of Dr. Gerry Shaw (University of Florida).
Immunofluorescent analysis of dissociated cell culture prepared from a neo-natal mouse brain labelling Neurofilament heavy polypeptide with ab134459 at 1/5000 dilution followed by Secondary antibody (fluorescein-labeled goat anti-chicken IgY used at 1/1000 dilution. Neurofilament heavy polypeptide (green staining) can be found within the neuronal cell body and in the neurites.
实验方案
数据表及文件
-
Datasheet download
文献 (2)
ab134459 被引用在 2 文献中.
- Szobota S et al. BDNF, NT-3 and Trk receptor agonist monoclonal antibodies promote neuron survival, neurite extension, and synapse restoration in rat cochlea ex vivo models relevant for hidden hearing loss. PLoS One 14:e0224022 (2019). PubMed: 31671109
- Park SH et al. Adeno-associated virus serotype rh10 is a useful gene transfer vector for sensory nerves that innervate bone in immunodeficient mice. Sci Rep 7:17428 (2017). PubMed: 29233995