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Neuroscience Neurotransmission Intracellular Signaling Cytoskeletal

Anti-Neurofibromin抗体(ab17963)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (14)

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Western blot - Anti-Neurofibromin antibody (ab17963)
  • Immunoprecipitation - Anti-Neurofibromin antibody (ab17963)

Key features and details

  • Rabbit polyclonal to Neurofibromin
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Neurofibromin抗体
    参阅全部 Neurofibromin 一抗
  • 描述

    兔多克隆抗体to Neurofibromin
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IPmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Cow, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, 293T, TCMK-1 and NIH3T3 whole cell lysates. IP: HeLa whole cell lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Cytoskeletal
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab17963于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
1/1000 - 1/10000. Predicted molecular weight: 319 kDa.
IP
Use a concentration of 2 - 10 µg/ml.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 319 kDa.
IP
Use a concentration of 2 - 10 µg/ml.

靶标

  • 功能

    Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
  • 疾病相关

    Defects in NF1 are the cause of neurofibromatosis type 1 (NF1) [MIM:162200]; also known as von Recklinghausen syndrome. A disease characterized by patches of skin pigmentation (cafe-au-lait spots), Lisch nodules of the iris, tumors in the peripheral nervous system and fibromatous skin tumors. Individuals with the disorder have increased susceptibility to the development of benign and malignant tumors.
    Defects in NF1 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. Germline mutations of NF1 account for the association of JMML with type 1 neurofibromatosis (NF1).
    Defects in NF1 are the cause of Watson syndrome (WS) [MIM:193520]. WS is characterized by the presence of pulmonary stenosis, cafe-au-lait spots, and mental retardation. WS is considered as an atypical form of NF1.
    Defects in NF1 are a cause of familial spinal neurofibromatosis (FSNF) [MIM:162210]. Familial spinal NF is considered to be an alternative form of neurofibromatosis, showing multiple spinal tumors.
    Defects in NF1 are a cause of neurofibromatosis-Noonan syndrome (NFNS) [MIM:601321]. NFNS is characterized by manifestations of both NF1 and Noonan syndrome (NS). NS is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis.
    Defects in NF1 may be a cause of colorectal cancer (CRC) [MIM:114500].
  • 序列相似性

    Contains 1 CRAL-TRIO domain.
    Contains 1 Ras-GAP domain.
  • Target information above from: UniProt accession P21359 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 280877 Cow
    • Entrez Gene: 4763 Human
    • Entrez Gene: 18015 Mouse
    • Entrez Gene: 24592 Rat
    • Omim: 613113 Human
    • SwissProt: P21359 Human
    • SwissProt: Q04690 Mouse
    • SwissProt: P97526 Rat
    • Unigene: 113577 Human
    see all
  • 别名

    • DKFZp686J1293 antibody
    • FLJ21220 antibody
    • Neurofibromatosis Noonan syndrome antibody
    • Neurofibromatosis related protein NF 1 antibody
    • Neurofibromatosis related protein NF1 antibody
    • neurofibromatosis type I antibody
    • Neurofibromatosis-related protein NF-1 antibody
    • Neurofibromin 1 antibody
    • Neurofibromin truncated antibody
    • Neurofibromin1 antibody
    • NF 1 antibody
    • NF antibody
    • NF1 antibody
    • NF1_HUMAN antibody
    • NFNS antibody
    • Type 1 Neurofibromatosis antibody
    • von Recklinghausen disease neurofibromin antibody
    • von Recklinghausen disease related protein VRNF antibody
    • VRNF antibody
    • WATS antibody
    • Watson disease related protein WSS antibody
    • Watson syndrome antibody
    • WSS antibody
    see all

图片

  • Western blot - Anti-Neurofibromin antibody (ab17963)
    Western blot - Anti-Neurofibromin antibody (ab17963)
    All lanes : Anti-Neurofibromin antibody (ab17963) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate in NETN lysis buffer
    Lane 2 : 293T whole cell lysate in NETN lysis buffer
    Lane 3 : Jurkat whole cell lysate in NETN lysis buffer
    Lane 4 : TCMK-1 whole cell lysate in NETN lysis buffer
    Lane 5 : NIH3T3 whole cell lysate in NETN lysis buffer

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 319 kDa


    Exposure time: 75 seconds
  • Immunoprecipitation - Anti-Neurofibromin antibody (ab17963)
    Immunoprecipitation - Anti-Neurofibromin antibody (ab17963)

    Whole cell lysate (0.5-1.0mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

    Lane 1: IP using rabbit anti-Neurofibromin antibody.
    Lane 2: IP using ab17963 at 6 µg per reaction.
    Lane 3: Control IgG.

    For western blotting, ab17963 was used at 1 µg/ml.

实验方案

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (14)

发表研究结果有使用 ab17963?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab17963 被引用在 14 文献中.

  • Auf der Maur P  et al. N-acetylcysteine overcomes NF1 loss-driven resistance to PI3Kα inhibition in breast cancer. Cell Rep Med 4:101002 (2023). PubMed: 37044095
  • Chen M  et al. Icariin Promotes Osteogenic Differentiation in a Cell Model with NF1 Gene Knockout by Activating the cAMP/PKA/CREB Pathway. Molecules 28:N/A (2023). PubMed: 37446790
  • Gogleva A  et al. Knowledge graph-based recommendation framework identifies drivers of resistance in EGFR mutant non-small cell lung cancer. Nat Commun 13:1667 (2022). PubMed: 35351890
  • Smith AE  et al. HER2?+?breast cancers evade anti-HER2 therapy via a switch in driver pathway. Nat Commun 12:6667 (2021). PubMed: 34795269
  • Casingal CR  et al. Identification of FMRP target mRNAs in the developmental brain: FMRP might coordinate Ras/MAPK, Wnt/ß-catenin, and mTOR signaling during corticogenesis. Mol Brain 13:167 (2020). PubMed: 33323119
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Neurofibromin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Primary AML cells)
Permeabilization
Yes - TritonX-100 in PBS 10min
Specification
Human Primary AML cells
Blocking step
Peroxidase Blocking Solution, BLOXALL as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 03 2021

Western blot abreview for Anti-Neurofibromin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (B16 cells)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycin gel)
Loading amount
40 µg
Specification
B16 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 11 2021

Western blot abreview for Anti-Neurofibromin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U937 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine gel)
Loading amount
40 µg
Specification
U937 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 31 2020

Western blot abreview for Anti-Neurofibromin antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole 3 day post fertilization zebrafish embryos)
Loading amount
50 µg
Specification
whole 3 day post fertilization zebrafish embryos
Gel Running Conditions
Reduced Denaturing (3-8% TA)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 09 2009

Western blot abreview for Anti-Neurofibromin antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphoblast cell line)
Specification
Lymphoblast cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 24 2006

Question

I would like the ab17963 and a credit note for the second antibody. I am sorry that it took so long to reply back. Thank you for all of your help.

Read More

Abcam community

Verified customer

Asked on Aug 28 2012

Answer

Thank you for contacting us. Your credit note ID is xxxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. As requested, I have also issued a free of charge replacement with the order number xxxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Abcam Scientific Support

回复于 Aug 28 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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