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重组RabMAb

重组Anti-Neurofascin抗体[EPR19003] - BSA and Azide free (ab271948)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
  • Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19003] to Neurofascin - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Neurofascin抗体[EPR19003] - BSA and Azide free
    参阅全部 Neurofascin 一抗

  • 描述

    兔单克隆抗体[EPR19003] to Neurofascin - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    IHC is recommended for mouse and rat only.

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Human brain and cerebellum lysates; Mouse and rat brain and spinal cord lysates. IHC-P: Mouse cerebrum and colon tissues. Rat cerebrum tissue.

  • 常规说明

    ab271948 is the carrier-free version of ab184377.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271948于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Detects a band of approximately 186, 155, 125, 75 kDa (predicted molecular weight: 150 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
Use at an assay dependent concentration. Detects a band of approximately 186, 155, 125, 75 kDa (predicted molecular weight: 150 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on mouse cerebrum is observed [PMID: 23198679].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184377).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm and membrane staining on myenteric nerve plexus, and negative on epithelium cells and smooth muscle cells of mouse colon.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184377).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on rat cerebrum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184377).
  • Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)
    Anti-Neurofascin antibody [EPR19003] - BSA and Azide free (ab271948)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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