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重组RabMAb

重组Anti-NeuroD1抗体[EPR4008] (ab109224)

Reviews (1) Submit a question References (16)
Western blot - Anti-NeuroD1 antibody [EPR4008] (ab109224)
  • Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR4008] (ab109224)
  • Anti-NeuroD1 antibody [EPR4008] (ab109224)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4008] to NeuroD1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

概述

  • 产品名称

    Anti-NeuroD1抗体[EPR4008]
    参阅全部 NeuroD1 一抗

  • 描述

    兔单克隆抗体[EPR4008] to NeuroD1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WBmore details
    不适用于: Flow Cyt or IHC-P

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Y79 and fetal brain lysates; ICC/IF: Mouse primary neuron cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109224于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/200.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
说明
ICC/IF
1/200.
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 40 kDa).
应用说明
Is unsuitable for Flow Cyt or IHC-P.

靶标

  • 功能

    Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.

  • 疾病相关

    Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.

  • 序列相似性

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • 翻译后修饰

    Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.

  • 细胞定位

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • atonal antibody
    • basic helix loop helix transcription factor antibody
    • BETA 2 antibody
    • Beta cell E box transactivator 2 antibody
    • BETA2 antibody
    • BHF 1 antibody
    • BHF1 antibody
    • bHLHa3 antibody
    • class A basic helix loop helix protein 3 antibody
    • Class A basic helix-loop-helix protein 3 antibody
    • MODY 6 antibody
    • MODY6 antibody
    • NDF1_HUMAN antibody
    • NeuroD antibody
    • NeuroD1 antibody
    • Neurogenic differentiation 1 antibody
    • Neurogenic differentiation factor 1 antibody
    • neurogenic helix loop helix protein NEUROD antibody
    • Neuronal differentiation 1 antibody
    see all

图片

  • Western blot - Anti-NeuroD1 antibody [EPR4008] (ab109224)
    Western blot - Anti-NeuroD1 antibody [EPR4008] (ab109224)
    All lanes : Anti-NeuroD1 antibody [EPR4008] (ab109224) at 1/1000 dilution

    Lane 1 : Y79 lysates
    Lane 2 : Fetal brain lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa
    Observed band size: 47 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR4008] (ab109224)
    Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR4008] (ab109224)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling NeuroD1 with ab109224 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in a little of mouse primary neuron cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 µg/mL) (Red). The nuclear counterstain was DAPI (Blue).

  • Anti-NeuroD1 antibody [EPR4008] (ab109224)
    Anti-NeuroD1 antibody [EPR4008] (ab109224)

文献 (16)

发表研究结果有使用 ab109224？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109224 被引用在 16 文献中.

  • Lee MC  et al. A multiplexed in vivo approach to identify driver genes in small cell lung cancer. Cell Rep 42:111990 (2023). PubMed: 36640300
  • Qin W  et al. VNN1 overexpression in pancreatic cancer cells inhibits paraneoplastic islet function by increasing oxidative stress and inducing β‑cell dedifferentiation. Oncol Rep 49:N/A (2023). PubMed: 37114564
  • Matsui S  et al. High mRNA expression of POU2F3 in small cell lung cancer cell lines predicts the effect of lurbinectedin. Thorac Cancer 13:1184-1192 (2022). PubMed: 35278040
  • Kelenis DP  et al. Inhibition of Karyopherin β1-Mediated Nuclear Import Disrupts Oncogenic Lineage-Defining Transcription Factor Activity in Small Cell Lung Cancer. Cancer Res 82:3058-3073 (2022). PubMed: 35748745
  • Olsen RR  et al. ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer. Genes Dev 35:847-869 (2021). PubMed: 34016693
View all Publications for this product

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Western blot abreview for Anti-NeuroD1 antibody [EPR4008]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (10% SDS-PAGE)
Sample
Human Cell lysate - whole cell (Lung cancer cells)
Specification
Lung cancer cells
Blocking step
Odyssey Blocking Buffer in TBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Oct 03 2014

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