AB109224

重组Anti-NeuroD1抗体[EPR4008]

Name: NeuroD1抗体[EPR4008] (ab109224)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109224
Price: 3222 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-NeuroD1 antibody [EPR4008]

RabMAb
Recombinant
了解详情

(1 Review)

(25 Publications)

Anti-NeuroD1 antibody [EPR4008] (ab109224) is a rabbit monoclonal antibody detecting NeuroD1 in Western Blot, ICC/IF. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

查看别名

BHLHA3, NEUROD, NEUROD1, Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR4008] (AB109224)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NeuroD1 antibody [EPR4008] (AB109224)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling NeuroD1 with ab109224 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing nuclear staining in a little of mouse primary neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 μg/mL) (Red). The nuclear counterstain was DAPI (Blue).

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Western blot - Anti-NeuroD1 antibody [EPR4008] (AB109224)

All lanes:

Western blot - Anti-NeuroD1 antibody [EPR4008] (ab109224) at 1/1000 dilution

Lane 1:

Y79 lysates at 10 µg

Lane 2:

Fetal brain lysates at 10 µg

Predicted band size: 40 kDa

Observed band size: 47 kDa

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不同偶联物与剂型 (7)

Carrier free

Anti-NeuroD1 antibody [EPR4008] - BSA and Azide free
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-NeuroD1 antibody [EPR4008]
660 APC

APC Anti-NeuroD1 antibody [EPR4008]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-NeuroD1 antibody [EPR4008]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-NeuroD1 antibody [EPR4008]
578 PE

PE Anti-NeuroD1 antibody [EPR4008]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-NeuroD1 antibody [EPR4008]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4008

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-NeuroD1 antibody [EPR4008] (ab109224) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of NeuroD1?
Anti-NeuroD1 [EPR4008] (ab109224) specifically detects a band for NeuroD1 (UniProt: Q13562) at a molecular weight of 40kDa.

Trusted by the scientific community
Anti-NeuroD1 [EPR4008] (ab109224) was first used in a scientific publication in 2011 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR4008] also available for your convenience: ab109224, Carrier free - ab239955, PE - ab318364, APC - ab318467, Alexa Fluor® 488 - ab318570, Alexa Fluor® 647 - ab318673, Alexa Fluor® 594 - ab318776, Alexa Fluor® 555 - ab318876

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NeuroD1 also referred to as Neurogenic Differentiation 1 is a basic helix-loop-helix transcription factor with a molecular mass of approximately 40 kDa. This protein is expressed mainly in the central nervous system as well as in the pancreas. NeuroD1 plays a role in activating transcription by binding to DNA sequences in specific target genes. This process involves dimerization with E proteins facilitating its function as a transcriptional activator.

Biological function summary

NeuroD1 influences neural development and differentiation. It is vital in converting undifferentiated neural precursors into mature neurons ensuring proper brain formation. NeuroD1 forms part of a transcriptional complex with other neural transcription factors which coordinates the expression of genes needed for neuron specification. Apart from neurogenesis it regulates insulin gene expression in pancreatic beta cells linking neural functions to endocrine regulation.

Pathways

NeuroD1 functions in the neurogenesis pathway and interacts closely with the Notch signaling pathway. It plays a role by influencing the expression of genes essential for neuronal lineage commitment. In the insulin signaling pathway NeuroD1 cooperates with other transcription factors like Pdx1 and MafA managing the expression of insulin and other important beta-cell genes. Interactions with proteins like E47 increase its regulatory effects on these pathways.

Defects in NeuroD1 function connect to conditions such as diabetes mellitus and medulloblastoma. In diabetes mellitus NeuroD1 mutations can disturb insulin gene regulation impacting glucose metabolism severely. The connection with Pdx1 further magnifies its involvement in pancreatic beta-cell function. In medulloblastoma aberrant expression or mutations in NeuroD1 affect neuronal differentiation leading to tumor development. NeuroD1 influences cellular pathways interacting with proteins such as Sox9 which contributes to oncogenic processes in neural tissues.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a transcriptional activator : mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).

See full target information NEUROD1

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文献 (25)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadw9296 PubMed40712017

2025

Neurons derived from NeuroD1-expressing astrocytes transition through transit-amplifying intermediates but lack functional maturity.

Applications

Unspecified application

Species

Unspecified reactive species

Fangbing Chen,Xi Liu,Xiaowen Zhong,Xiaoqing Chen,Eva Nicholson,Kaiyi Liu,Huiyao Chen,Yifeng Lin,Yousheng Shu,Wenhao Zhou,Carol J Schuurmans,Q Richard Lu

Nucleic acids research 53: PubMed40548942

2025

The lincRNA Pantr1 is a FOXG1 target gene conferring site-specific chromatin binding of FOXG1.

Applications

Unspecified application

Species

Unspecified reactive species

Fabian Gather,Tudor Rauleac,Ipek Akol,Ganeshkumar Arumugam,Camila L Fullio,Teresa Müller,Dimitrios Kleidonas,Ruth Geiss-Friedlander,Andre Fischer,Andreas Vlachos,Rolf Backofen,Tanja Vogel

Molecular medicine (Cambridge, Mass.) 31:232 PubMed40500731

2025

ONECUT2 reprograms neuroendocrine fate and is an actionable therapeutic target in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mirian Gutiérrez,Irene Zamora,Raquel Iriarte,María José Pajares,Qian Yang,Chen Qian,Nerea Otegui,Joaquín Fernández-Irigoyen,Enrique Santamaría,Nicolas Alcala,Alexandra Sexton-Oates,Lynnette Fernández-Cuesta,Miguel Barajas,Alfonso Calvo,Luis M Montuenga,Beatrice Knudsen,Sungyong You,Michael R Freeman,Ignacio Encío,Mirja Rotinen

FEBS letters 599:2504-2522 PubMed40377017

2025

E3 ubiquitin ligase RMND5A maintains the self-renewal state of human neural stem/precursor cells by regulating Wnt and mTOR signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Takumi Nakagawa,Kosuke Hata,Yoshihiro Izumi,Hideyuki Nakashima,Sayako Katada,Taito Matsuda,Takeshi Bamba,Kinichi Nakashima

Cancer cell 42:1352-1369.e13 PubMed39029464

2024

Mammalian SWI/SNF complex activity regulates POU2F3 and constitutes a targetable dependency in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Leslie Duplaquet,Kevin So,Alexander W Ying,Shreoshi Pal Choudhuri,Xinyue Li,Grace D Xu,Yixiang Li,Xintao Qiu,Rong Li,Shilpa Singh,Xiaoli S Wu,Seth Hamilton,Victor D Chien,Qi Liu,Jun Qi,Tim D D Somerville,Hillary M Heiling,Emanuele Mazzola,Yenarae Lee,Thomas Zoller,Christopher R Vakoc,John G Doench,William C Forrester,Tinya Abrams,Henry W Long,Matthew J Niederst,Benjamin J Drapkin,Cigall Kadoch,Matthew G Oser

Methods in molecular biology (Clifton, N.J.) 2806:117-138 PubMed38676800

2024

A Multiplexed Approach to Assess Small Cell Lung Cancer Subtype Heterogeneity in Primary and Patient-Derived Tumor Samples.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Ng,Emma Pan,Alex Johnston,Nina Tubau Ribera,Ariena Kersbergen,Jonas B Hess,Sarah A Best,Ellen Tsui,Daniel Steinfort,Kate D Sutherland

Cancer cell 41:2066-2082.e9 PubMed37995683

2023

Temporal evolution reveals bifurcated lineages in aggressive neuroendocrine small cell prostate cancer trans-differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Chun Chen,Wendy Tran,Kai Song,Tyler Sugimoto,Matthew B Obusan,Liang Wang,Katherine M Sheu,Donghui Cheng,Lisa Ta,Grigor Varuzhanyan,Arthur Huang,Runzhe Xu,Yuanhong Zeng,Amirreza Borujerdpur,Nicholas A Bayley,Miyako Noguchi,Zhiyuan Mao,Colm Morrissey,Eva Corey,Peter S Nelson,Yue Zhao,Jiaoti Huang,Jung Wook Park,Owen N Witte,Thomas G Graeber

Oncology reports 49: PubMed37114564

2023

VNN1 overexpression in pancreatic cancer cells inhibits paraneoplastic islet function by increasing oxidative stress and inducing β‑cell dedifferentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Qin,Muxing Kang,Chao Li,Wen Zheng,Qingqu Guo

Cell reports 42:111990 PubMed36640300

2023

A multiplexed in vivo approach to identify driver genes in small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Myung Chang Lee,Hongchen Cai,Christopher W Murray,Chuan Li,Yan Ting Shue,Laura Andrejka,Andy L He,Alessandra M E Holzem,Alexandros P Drainas,Julie H Ko,Garry L Coles,Christina Kong,Shirley Zhu,ChunFang Zhu,Jason Wang,Matt van de Rijn,Dmitri A Petrov,Monte M Winslow,Julien Sage

Cancer cell 40:1423-1439.e11 PubMed36240778

2022

Spatial epitope barcoding reveals clonal tumor patch behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Xavier Rovira-Clavé,Alexandros P Drainas,Sizun Jiang,Yunhao Bai,Maya Baron,Bokai Zhu,Alec E Dallas,Myung Chang Lee,Theresa P Chu,Alessandra Holzem,Ramya Ayyagari,Debadrita Bhattacharya,Erin F McCaffrey,Noah F Greenwald,Maxim Markovic,Garry L Coles,Michael Angelo,Michael C Bassik,Julien Sage,Garry P Nolan

View all publications

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