Anti-NeuroD1抗体[3H8] - BSA and Azide free (ab60704)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)](https://www.abcam.cn/ps/products/60/ab60704/Images/ab60704-251134-anti-neurod1-antibody-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)")
Key features and details
- Mouse monoclonal [3H8] to NeuroD1 - BSA and Azide free
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-NeuroD1抗体[3H8] - BSA and Azide free
参阅全部 NeuroD1 一抗 -
描述
小鼠单克隆抗体[3H8] to NeuroD1 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, Flow Cytmore details -
种属反应性
与反应: Human
预测可用于: Rat, Sheep, Cow
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免疫原
Recombinant fragment with tag: QDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS AEFEKNYAFT , corresponding to amino acids 201-300 of Human NeuroD1
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阳性对照
- IMR-32 (human neuroblastoma) whole cell lysate and human ovary, clear cell carcinoma tissue.
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常规说明
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
3H8 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab60704于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (5) |
Use at an assay dependent concentration.
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| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
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Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box. -
疾病相关
Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4760 Human
- Entrez Gene: 29458 Rat
- Omim: 601724 Human
- SwissProt: Q13562 Human
- SwissProt: Q64289 Rat
- Unigene: 574626 Human
- Unigene: 709709 Human
- Unigene: 44289 Rat
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别名
- atonal antibody
- basic helix loop helix transcription factor antibody
- BETA 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)This image is courtesy of an anonymous Abreview.
Immunohistochemical analysis of formaldehyde fixed human cerebellum sections incubated with ab60704 for 20 minutes at 25°C in a concentration of 1/400. The blocking step was performed with 3% H2O2 for 10 minutes at 25ºC. The secondary antibody used was a polyclonal goat anti-mouse/rabbit HRP conjugate, used undiluted.
This image was generated using the ascites version of the product.
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![Western blot - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)](https://www.abcam.cn/ps/products/60/ab60704/Images/ab60704-74227-anti-neurod1-antibody-western-blot.jpg)
Western blot - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704) + IMR-32 (Human Caucasian neuroblastoma) whole cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)](https://www.abcam.cn/ps/products/60/ab60704/Images/ab60704-74226-anti-neurod1-antibody-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)ab60704 at 3ug/ml staining NeuroD1 in human ovary, clear cell carcinoma by Immunohistochemistry, Formalin-fixed Paraffin-embedded tissue.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)](https://www.abcam.cn/ps/products/60/ab60704/Images/ab60704-74223-anti-neurod1-antibody-flow-cytometry.jpg)
Flow Cytometry - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)Overlay histogram showing SHSY-5Y cells stained with ab60704 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60704, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (59)
ab60704 被引用在 59 文献中.
- Heise J et al. Protective Effects of Early Caffeine Administration in Hyperoxia-Induced Neurotoxicity in the Juvenile Rat. Antioxidants (Basel) 12:N/A (2023). PubMed: 36829854
- Chen X et al. MicroRNA-375 Is Induced during Astrocyte-to-Neuron Reprogramming and Promotes Survival of Reprogrammed Neurons when Overexpressed. Cells 12:N/A (2023). PubMed: 37681934
- Sanghrajka RM et al. KMT2D suppresses Sonic hedgehog-driven medulloblastoma progression and metastasis. iScience 26:107831 (2023). PubMed: 37822508
- Nakao-Ise Y et al. Induction of MYCN-amplified neuroblastoma differentiation through NMYC suppression using PPAR-γ antagonist. J Clin Biochem Nutr 73:191-197 (2023). PubMed: 37970556
- Anastasaki C et al. Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization. STAR Protoc 3:101173 (2022). PubMed: 35199037
