Anti-Neurocan抗体[650.24] (ab31979)
Key features and details
- Mouse monoclonal [650.24] to Neurocan
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Neurocan抗体[650.24]
参阅全部 Neurocan 一抗 -
描述
小鼠单克隆抗体[650.24] to Neurocan -
宿主
Mouse -
特异性
Specific for Neurocan core protein; also reacts with neurocan C fragment -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Tissue, cells or virus corresponding to Rat Neurocan.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
650.24 -
骨髓瘤
P3x63 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31979于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (4) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
|
说明 |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
靶标
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功能
May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. -
组织特异性
Brain. -
序列相似性
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 1463 Human
- Entrez Gene: 13004 Mouse
- Entrez Gene: 58982 Rat
- Omim: 600826 Human
- SwissProt: O14594 Human
- SwissProt: P55066 Mouse
- SwissProt: P55067 Rat
- Unigene: 169047 Human
see all -
别名
- Chondroitin sulfate proteoglycan 3 antibody
- Cspg3 antibody
- Ncan antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Neurocan antibody [650.24] (ab31979)Image courtesy of an anonymous Abreview.ab31979 staining Neurocan in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab31979 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-mouse polyclonal, used at a 1/300 dilution. Nuclei are counterstained with DAPI.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab31979 被引用在 4 文献中.
- Wei J et al. Spatiotemporal proteomic atlas of multiple brain regions across early fetal to neonatal stages in cynomolgus monkey. Nat Commun 14:3917 (2023). PubMed: 37400444
- Su H et al. TMAO Aggregates Neurological Damage Following Ischemic Stroke by Promoting Reactive Astrocytosis and Glial Scar Formation via the Smurf2/ALK5 Axis. Front Cell Neurosci 15:569424 (2021). PubMed: 33815059
- Huang X et al. Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury. CNS Neurosci Ther N/A:N/A (2020). PubMed: 32449258
- Li G et al. Mdivi-1 Inhibits Astrocyte Activation and Astroglial Scar Formation and Enhances Axonal Regeneration after Spinal Cord Injury in Rats. Front Cell Neurosci 10:241 (2016). WB, ICC/IF ; Rat . PubMed: 27807407