Anti-NEURL1B抗体(ab122400)
Key features and details
- Rabbit polyclonal to NEURL1B
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-NEURL1B抗体 -
描述
兔多克隆抗体to NEURL1B -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen: ALIDVYGITD EVQLLESAFA DTLTPARLSQ ARFSACLPPS SHDAANFDNN ELENNQVVAK LGHL, corresponding to amino acids 178-241 of Human NEURL1B according to A8MQ27
-
阳性对照
- Human rectum tissue; Human liver, Human tonsil, RT-4 and U-251 MG cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122400于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
-
功能
E3 ubiquitin-protein ligase. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 2 NHR (neuralized homology repeat) domains.
Contains 1 RING-type zinc finger. - Information by UniProt
-
数据库链接
- Entrez Gene: 54492 Human
- SwissProt: A8MQ27 Human
- Unigene: 91521 Human
-
别名
- E3 ubiquitin-protein ligase NEURL1B antibody
- E3 ubiquitin-protein ligase NEURL3 antibody
- hNeur2 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-NEURL1B antibody (ab122400)Immunofluorescent staining of Human cell line U-251 MG shows positivity in vesicles. Recommended concentration of ab122400 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEURL1B antibody (ab122400)ab122400, at a 1/225 dilution, staining NEURL1B in glandular cells of paraffin-embedded Human rectum tissue by immunohistochemistry. -
Western blot - Anti-NEURL1B antibody (ab122400)All lanes : Anti-NEURL1B antibody (ab122400) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab122400 尚未被引用在任何文献中。