Anti-NeuN抗体- Neuronal Marker (ab134014)
Key features and details
- Chicken polyclonal to NeuN - Neuronal Marker
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgY
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-NeuN抗体- Neuronal Marker
参阅全部 NeuN 一抗 -
描述
鸡多克隆抗体to NeuN - Neuronal Marker -
宿主
Chicken -
特异性
We recommend ab177487 as an alternative for IHC-P and WB
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经测试应用
适用于: IHC-P, WBmore details
不适用于: IHC-Fr -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to NeuN (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). Corresponding to a region within Human (NP_001076044) and Mouse (NP_001020102).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.9% Sodium chloride -
Concentration information loading...
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纯度
Affinity purified -
纯化说明
ab134014 was affinity-purified from the IgY fraction prepared from eggs laid by immunised hens. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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ELISA pair antibody
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134014于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (3) |
1/100.
NeuN is a nuclear antigen and ab134014 is a chicken antibody, which has a slightly higher molecular weight compared to IgG. We recommend performing heat mediated antigen retrieval with Citrate buffer pH 6.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. |
WB |
1/100. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).Can be blocked with Alexa Fluor® 594 Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (ab207279).
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说明 |
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IHC-P
1/100. NeuN is a nuclear antigen and ab134014 is a chicken antibody, which has a slightly higher molecular weight compared to IgG. We recommend performing heat mediated antigen retrieval with Citrate buffer pH 6.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. |
WB
1/100. Detects a band of approximately 42 kDa (predicted molecular weight: 34 kDa).Can be blocked with Alexa Fluor® 594 Rabbit monoclonal [EPR12763] to NeuN - Neuronal Marker (ab207279). |
靶标
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功能
RNA-binding protein that regulates alternative splicing events. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 146713 Human
- Entrez Gene: 52897 Mouse
- Entrez Gene: 287847 Rat
- SwissProt: A6NFN3 Human
- SwissProt: Q8BIF2 Mouse
- Unigene: 135229 Human
- Unigene: 341103 Mouse
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别名
- FLJ56884 antibody
- FLJ58356 antibody
- Fox-1 homolog C antibody
see all
图片
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All lanes : Anti-NeuN antibody - Neuronal Marker (ab134014) at 1/1000 dilution
Lane 1 : Mouse Brain
Lane 2 : Rat Brain
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse cerebral cortex tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab134014)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse CA1 hippocampus tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab134014 被引用在 24 文献中.
- Galán-Llario M et al. Receptor protein tyrosine phosphatase β/ζ regulates loss of neurogenesis in the mouse hippocampus following adolescent acute ethanol exposure. Neurotoxicology 94:98-107 (2023). PubMed: 36402194
- Kapell H et al. Neuron-oligodendrocyte potassium shuttling at nodes of Ranvier protects against inflammatory demyelination. J Clin Invest 133:N/A (2023). PubMed: 36719741
- Beckmann A et al. Moesin is an effector of tau-induced actin overstabilization, cell cycle activation, and neurotoxicity in Alzheimer's disease. iScience 26:106152 (2023). PubMed: 36879821
- Okada T et al. Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target. Sci Adv 7:N/A (2021). PubMed: 33741588
- Yoon Y et al. PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival. Mol Psychiatry 26:1996-2012 (2021). PubMed: 32541930