Anti-Nestin抗体- Neural Stem Cell Marker (ab7659)
Key features and details
- Rabbit polyclonal to Nestin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Nestin抗体- Neural Stem Cell Marker
参阅全部 Nestin 一抗 -
描述
兔多克隆抗体to Nestin -
宿主
Rabbit -
特异性
Detects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB.
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经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse
预测可用于: Rat, Rabbit, Hamster
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免疫原
Synthetic peptide:
ALETESQDSAEPSGSEE
, corresponding to amino acids 1481-1497 of Human Nestin. -
常规说明
ab7659 has been upgraded from Fast Track status based on data obtained in a new batch of this antibody.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This affinity purified antibody is directed against human Nestin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000.
Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview). |
| 说明 |
|---|
|
WB
1/500 - 1/3000. Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview). |
应用说明
Is unsuitable for ICC/IF or IHC-P.
靶标
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功能
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
组织特异性
CNS stem cells. -
序列相似性
Belongs to the intermediate filament family. -
发展阶段
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
翻译后修饰
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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数据库链接
- Entrez Gene: 18008 Mouse
- SwissProt: Q6P5H2 Mouse
- SwissProt: P21263 Rat
- Unigene: 331129 Mouse
- Unigene: 9701 Rat
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别名
- ESTM 46 antibody
- FLJ 21841 antibody
- FLJ21841 antibody
see all
图片
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Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1/2000 dilution + MEF whole cell lysate at 30 µg
Secondary
IRDye 800
conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 200 kDa why is the actual band size different from the predicted? -
Western blot - Anti-Nestin antibody - Neural Stem Cell Marker (ab7659)This image is courtesy of Randal Moldrich, CNRS UMR7637, ESPCI, FranceAll lanes : Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1 µg/ml
Lane 1 : Mouse neural precursors whole cell lysate reduced but not denatured
Lane 2 : Mouse neural precursors whole cell lysate reduced and denatured
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 230 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
A slightly higher band obtained than expected, but as predicted in the datasheet. Denaturing of the WB lysate results in a loss of Nestin specific immunoreactivity. NB: Equal protein loading was verified.
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab7659 被引用在 17 文献中.
- Kim W et al. Suppression of transient receptor potential melastatin 7 regulates pluripotency, proliferation, and differentiation of mouse embryonic stem cells via mechanistic target of rapamycin-extracellular signal-regulated kinase activation. J Cell Biochem 123:547-567 (2022). PubMed: 34958137
- Mollichella ML et al. Isolation and Characterization of Cat Olfactory Ecto-Mesenchymal Stem Cells. Animals (Basel) 12:N/A (2022). PubMed: 35625130
- Okawa ER et al. Essential roles of insulin and IGF-1 receptors during embryonic lineage development. Mol Metab 47:101164 (2021). PubMed: 33453419
- Fan X et al. TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation. Elife 10:N/A (2021). PubMed: 33554859
- Sun X et al. ADNP promotes neural differentiation by modulating Wnt/ß-catenin signaling. Nat Commun 11:2984 (2020). PubMed: 32533114