Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
使用敲除细胞株进行验证

Anti-Nestin抗体[2C1.3A11] (ab18102)

Reviews (1)Q&A (1)References (28)
Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [2C1.3A11] (ab18102)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
  • Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
  • Flow Cytometry - Anti-Nestin antibody [2C1.3A11] (ab18102)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)

Key features and details

  • Mouse monoclonal [2C1.3A11] to Nestin
  • Suitable for: ICC/IF, WB, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

FITC

概述

  • 产品名称

    Anti-Nestin抗体[2C1.3A11]
    参阅全部 Nestin 一抗

  • 描述

    小鼠单克隆抗体[2C1.3A11] to Nestin

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, WB, Flow Cyt, IHC-Pmore details

  • 种属反应性

    与反应: Human
    不与反应: Rat

  • 免疫原

    Recombinant fragment corresponding to Human Nestin aa 1450-1650.

  • 常规说明


    This highly specific antibody to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab18102于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100 - 1/500.
WB
1/1000 - 1/5000. Predicted molecular weight: 177 kDa.
Flow Cyt (1)
1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.
说明
ICC/IF
1/100 - 1/500.
WB
1/1000 - 1/5000. Predicted molecular weight: 177 kDa.
Flow Cyt
1/200.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

  • 组织特异性

    CNS stem cells.

  • 序列相似性

    Belongs to the intermediate filament family.

  • 发展阶段

    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

  • 翻译后修饰

    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    图片

    • Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)
      All lanes : Anti-Nestin antibody [2C1.3A11] (ab18102) at 1/5000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : NES knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 177 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab18102 observed at 300 kDa. Red - loading control, ab176560, observed at 50 kDa.

      ab18102 was shown to recognize NES in wild-type HAP1 cells as signal was lost at the expected MW in NES knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NES knockout samples were subjected to SDS-PAGE. Ab18102 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunocytochemistry/ Immunofluorescence - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunofluorescence staining of U251 cells (glioblastoma cell line) stained using ab18102(green) and bisbenzimide (blue)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of migrating tumor cells labelling Nestin with ab18102 at 2µg/ml. Cytoplasm and membrane positive staining shown.

    • Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Western blot - Anti-Nestin antibody [2C1.3A11] (ab18102)
      All lanes : Anti-Nestin antibody [2C1.3A11] (ab18102) at 1/1000 dilution

      Lane 1 : Total rat brain protein at 5 µg
      Lane 2 : Human CNS progenitor cell protein at 1 µg
      Lane 3 : Human CNS progenitor cell protein at 5 µg

      Predicted band size: 177 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of xenograft tumor cells labelling Nestin with ab18102.

    • Flow Cytometry - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Flow Cytometry - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Overlay histogram showing SH-SY5Y cells stained with ab18102 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18102, 1/200 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nestin antibody [2C1.3A11] (ab18102)
      Immunohistochemistry of Anti-Nestin on formalin fixed paraffin embedded prostate nerve tissue using ab18102

    文献 (28)

    发表研究结果有使用 ab18102？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab18102 被引用在 28 文献中.

    • Kawatani K  et al. ABCA7 deficiency causes neuronal dysregulation by altering mitochondrial lipid metabolism. Mol Psychiatry 29:809-819 (2024). PubMed: 38135757
    • Grunwald V  et al. Development of Zika Virus E Variants for Pseudotyping Retroviral Vectors Targeting Glioblastoma Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37833934
    • Zhao J  et al. APOE deficiency impacts neural differentiation and cholesterol biosynthesis in human iPSC-derived cerebral organoids. Stem Cell Res Ther 14:214 (2023). PubMed: 37605285
    • Tian G  et al. rAAV2-Mediated Restoration of GALC in Neural Stem Cells from Krabbe Patient-Derived iPSCs. Pharmaceuticals (Basel) 16:N/A (2023). PubMed: 37111381
    • Azevedo-Pereira RL  et al. Decoding the molecular crosstalk between grafted stem cells and the stroke-injured brain. Cell Rep 42:112353 (2023). PubMed: 37043353
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Flow Cytometry abreview for Anti-Nestin antibody [2C1.3A11] - Neural Stem Cell Marker

     Excellent
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Neural Progenitor cells (from human ES cells))
    Specification
    Neural Progenitor cells (from human ES cells)
    Preparation
    Cell harvesting/tissue preparation method: Cell Dissociation Buffer
    Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 70% Methanol (ice cold)
    Gating Strategy
    FSC vs SSC on negative control sample
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jan 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com