Anti-nephronectin抗体(ab272549)
Key features and details
- Rabbit polyclonal to nephronectin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-nephronectin抗体 -
描述
兔多克隆抗体to nephronectin -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Orangutan
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免疫原
Recombinant fragment corresponding to Human nephronectin aa 150-350.
Database link: Q6UXI9 -
阳性对照
- IHC-P: Human thyroid tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272549于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins. -
组织特异性
Expressed in kidney and lung and to a lower extent in brain, pregnant uterus, placenta, thyroid gland and blood vessels. -
序列相似性
Belongs to the nephronectin family.
Contains 5 EGF-like domains.
Contains 1 MAM domain. -
发展阶段
Expressed in fetal kidney, cochlea, eye, heart and lung. -
结构域
The MAM domain is required for localization at the cell surface. -
细胞定位
Secreted > extracellular space > extracellular matrix. Trapped on the cell surface or in the extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 255743 Human
- Omim: 610306 Human
- SwissProt: Q6UXI9 Human
- Unigene: 518921 Human
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别名
- EGFL6L antibody
- Nephronectin antibody
- NPNT antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nephronectin antibody (ab272549)
Paraffin-embedded formalin-fixed human thyroid tissue stained for nephronectin using ab272549 at 1/20 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272549 被引用在 1 文献中.
- Hong W et al. BRG1 Mediates Nephronectin Activation in Hepatocytes to Promote T Lymphocyte Infiltration in ConA-Induced Hepatitis. Front Cell Dev Biol 8:587502 (2020). PubMed: 33553140