Anti-Nephrin (phospho Y1176 + Y1193)抗体[EPTPG1] (ab80299)
![Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)](https://www.abcam.cn/ps/products/80/ab80299/Images/ab80299-3-ab80299WB1.jpg "Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)")
Key features and details
- Rabbit monoclonal [EPTPG1] to Nephrin (phospho Y1176 + Y1193)
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Nephrin (phospho Y1176 + Y1193)抗体[EPTPG1]
参阅全部 Nephrin 一抗 -
描述
兔单克隆抗体[EPTPG1] to Nephrin (phospho Y1176 + Y1193) -
宿主
Rabbit -
特异性
This antibody detects Nephrin when either tyrosines 1176 or 1193 are phosphorylated, but does not detect non-phosphorylated Nephrin. -
经测试应用
适用于: WB, Dot blotmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Transfected HEK293T cells
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPTPG1 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80299于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 138, 180 kDa (predicted molecular weight: 138 kDa).
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| Dot blot |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 138, 180 kDa (predicted molecular weight: 138 kDa). |
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Dot blot
Use at an assay dependent concentration. |
靶标
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功能
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. -
组织特异性
Specifically expressed in podocytes of kidney glomeruli. -
疾病相关
Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. -
序列相似性
Belongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains. -
发展阶段
In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli. -
翻译后修饰
Phosphorylated on tyrosine residues. -
细胞定位
Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4868 Human
- Entrez Gene: 54631 Mouse
- Omim: 602716 Human
- SwissProt: O60500 Human
- SwissProt: Q9QZS7 Mouse
- Unigene: 122186 Human
- Unigene: 437830 Mouse
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别名
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
see all
图片
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Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/1000 dilution
Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin expression vector, whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate
Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 138 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)](https://www.abcam.cn/ps/products/80/ab80299/Images/ab80299-2-ab80299Dot1.jpg)
Dot Blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)Dot blot analysis of Nephrin (pY1176 + pY1193) phospho peptide (Lane 1), Nephrin (pY1176) phospho peptide (Lane 2), Nephrin (pY1193) phospho peptide (Lane 3) and Nephrin non-phospho peptide (Lane 4) labelling Nephrin (pY1176 + pY1193) with ab80299 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)](https://www.abcam.cn/ps/products/80/ab80299/Images/Nephrin-Primary-antibodies-ab80299-1.jpg)
Western blot - Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/50000 dilution
Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin expression vector, whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1176F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Lane 4 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1193F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 138 kDa
Observed band size: 138,180 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab80299 被引用在 10 文献中.
- Jiang W et al. Klotho inhibits renal ox-LDL deposition via IGF-1R/RAC1/OLR1 signaling to ameliorate podocyte injury in diabetic kidney disease. Cardiovasc Diabetol 22:293 (2023). PubMed: 37891556
- Jansen J et al. Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling. Development 149:N/A (2022). PubMed: 35417019
- Cara-Fuentes G et al. Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood. iScience 25:104694 (2022). PubMed: 35847557
- Solanki AK et al. Phosphorylation of slit diaphragm proteins NEPHRIN and NEPH1 upon binding of HGF promotes podocyte repair. J Biol Chem 297:101079 (2021). PubMed: 34391780
- Martin CE et al. Multivalent nephrin-Nck interactions define a threshold for clustering and tyrosine-dependent nephrin endocytosis. J Cell Sci 133:N/A (2020). PubMed: 31974115
- Chun J et al. Recruitment of APOL1 kidney disease risk variants to lipid droplets attenuates cell toxicity. Proc Natl Acad Sci U S A 116:3712-3721 (2019). PubMed: 30733285
- Wei C et al. SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts. J Am Soc Nephrol 29:2641-2657 (2018). PubMed: 30341149
- Lin JS et al. ARF6 mediates nephrin tyrosine phosphorylation-induced podocyte cellular dynamics. PLoS One 12:e0184575 (2017). PubMed: 28880939
- Kapodistria K et al. Nephrin, a transmembrane protein, is involved in pancreatic beta-cell survival signaling. Mol Cell Endocrinol 400:112-28 (2015). PubMed: 25448064
- Jeon J et al. Dynamin-mediated Nephrin Phosphorylation Regulates Glucose-stimulated Insulin Release in Pancreatic Beta Cells. J Biol Chem 287:28932-42 (2012). WB ; Human . PubMed: 22718751