AB216341

重组Anti-Nephrin抗体[EPR20993]

Name: Nephrin抗体[EPR20993] (ab216341)| Abcam中文官网
Brand: Abcam Limited
SKU: AB216341
Price: 2679 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-Nephrin antibody [EPR20993]

RabMAb
Recombinant
了解详情

(1 Review)

(90 Publications)

Anti-Nephrin antibody [EPR20993] (ab216341) is a rabbit monoclonal antibody detecting Nephrin in Western Blot, IP, IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications

查看别名

NPHN, NPHS1, Nephrin, Renal glomerulus-specific cell adhesion receptor

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody [EPR20993] (AB216341)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody [EPR20993] (AB216341)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Nephrin with ab216341 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human renal glomerulus (PMID : 18599604; PMID : 25367693). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Primary antibody incubation overnight at +4°C is recommended for human species

Immunohistochemistry (Frozen sections) - Anti-Nephrin antibody [EPR20993] (AB216341)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nephrin antibody [EPR20993] (AB216341)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney tissue labeling Nephrin with ab216341 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the glomerular podocytes (PMID : 26380915).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody [EPR20993] (AB216341)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Nephrin with ab216341 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on rat renal glomerulus (PMID : 18599604; PMID : 25367693). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nephrin antibody [EPR20993] (AB216341)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Nephrin with ab216341 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse renal glomerulus (PMID : 18599604; PMID : 25367693). Counter stained with hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Nephrin antibody [EPR20993] (AB216341)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat kidney tissue labeling Nephrin with ab216341 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the glomerular podocytes (PMID : 26380915).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-Nephrin antibody [EPR20993] (AB216341)

Nephrin was immunoprecipitated from 0.35 mg of mouse kidney lysate with ab216341 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216341 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : Mouse kidney lysate 10 μg (Input).

Lane 2 : ab216341 IP in mouse kidney lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab216341 in mouse kidney lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

All lanes:

Immunoprecipitation - Anti-Nephrin antibody [EPR20993] (ab216341)

Predicted band size: 134 kDa

false

Supplier Data

Western blot - Anti-Nephrin antibody [EPR20993] (AB216341)

Blocking/Dilution buffer : 5% NFDM/TBST.

Use high sensitivity ECL to obtain a strong signal.

All lanes:

Western blot - Anti-Nephrin antibody [EPR20993] (ab216341) at 1/1000 dilution

All lanes:

Rat kidney lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 134 kDa

Observed band size: 200 kDa

true

Exposure time: 15s

Supplier Data

Western blot - Anti-Nephrin antibody [EPR20993] (AB216341)

Blocking/Dilution buffer : 5% NFDM/TBST.

The MW is consistent with the literature (PMID 11733379).

All lanes:

Western blot - Anti-Nephrin antibody [EPR20993] (ab216341) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 134 kDa

Observed band size: 200 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-Nephrin antibody [EPR20993] (AB216341)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Nephrin antibody [EPR20993] (ab216341) at 1/10000 dilution

All lanes:

His-tagged mouse nephrin (aa1001-1250) recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 134 kDa

Observed band size: 38 kDa

true

Exposure time: 15s

CiteAb

Western blot - Anti-Nephrin antibody [EPR20993] (AB216341)

Nephrin western blot using anti-Nephrin antibody [EPR20993] ab216341. Publication image and figure legend from Gong, Q., He, L. L., et al., 2019, Evid Based Complement Alternat Med, PubMed 31275420.

ab216341 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab216341 please see the product overview.

Fibrosis of kidney and expressions of the factors after the intravenous administration of B4. (a)–(f) Morphology of kidney (200×amplification). (a) normal; (b) model; (c) prednisolone (Predn.); (d) B4 (2.5 mg/kg); (e) B4 (1.25 mg/kg); (f) B4 (0.625 mg/kg); (g) the statistical analysis of the fibrosis in the kidney images by using grayscale scanning. (h)-(j) Protein expression of nephrin and podocin. The data were shown as mean ± SEM from 10 rats in each group. ##, compared with the normal control, p < 0.01. *, **, compared with model groups, p < 0.05 & p < 0.01. NS means no significance.

false

不同偶联物与剂型 (7)

Carrier free

Anti-Nephrin antibody [EPR20993] - BSA and Azide free
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Nephrin antibody [EPR20993]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Nephrin antibody [EPR20993]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Nephrin antibody [EPR20993]
660 APC

APC Anti-Nephrin antibody [EPR20993]
578 PE

PE Anti-Nephrin antibody [EPR20993]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Nephrin antibody [EPR20993]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20993

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IHC-P, IHC-Fr, WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "Use 1/2000 dilution for Mouse and Rat.Primary antibody incubation overnight at +4°C is recommended for human species. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "Use 1/2000 dilution for Mouse and Rat.Primary antibody incubation overnight at +4°C is recommended for human species. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "Use 1/2000 dilution for Mouse and Rat.Primary antibody incubation overnight at +4°C is recommended for human species. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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产品详情

What is this antibody validated in?
Anti-Nephrin antibody [EPR20993] (ab216341) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.

What is the molecular weight of Nephrin?
Anti-Nephrin [EPR20993] (ab216341) specifically detects a band for Nephrin (UniProt: Q9QZS7) at a molecular weight of 136kDa.

Trusted by the scientific community
Anti-Nephrin [EPR20993] (ab216341) was first used in a scientific publication in 2017 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR20993] also available for your convenience: ab216341, Carrier free - ab227806, PE - ab318357, APC - ab318460, Alexa Fluor^® 488 - ab318563, Alexa Fluor^® 594 - ab318769, Alexa Fluor^® 555 - ab318869, Alexa Fluor^® 750 - ab321387

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Nephrin also known as NPHS1 is a transmembrane protein with a molecular weight of approximately 180 kDa. Researchers have predominantly observed nephrin in the podocytes of the kidney glomerulus. Nephrin plays a mechanical role as part of the slit diaphragm a structure critical for maintaining the filtration barrier of the kidney. Its structure includes multiple immunoglobulin-like domains which facilitate cell signaling and adhesion processes important for podocyte function.

Biological function summary

Nephrin is essential for the regulation of glomerular permeability and podocyte foot process architecture. It serves as a significant component of the slit diaphragm complex working alongside proteins like podocin and CD2AP. This protein complex helps regulate the integrity and selectivity of the glomerular filtration barrier ensuring that blood plasma contents are selectively filtered while preventing the loss of large proteins into urine.

Pathways

Nephrin is integral to the PI3K/Akt signaling pathway influencing cell survival and cytoskeletal organization in podocytes. Nephrin's interaction within this pathway involves related proteins such as GAPDH and PTPRO which impact cellular functions critical to maintaining kidney structure and function. Additionally nephrin participates in the synaptopodin pathway modulating actin dynamics and therefore playing a role in cellular contraction and movement.

Nephrin dysfunction is closely related to congenital nephrotic syndrome particularly Finnish-type nephrosis which results in significant proteinuria and kidney failure. Nephrin mutations or aberrant expression can also contribute to other glomerular diseases such as focal segmental glomerulosclerosis (FSGS). In these conditions nephrin's relationship with other proteins like neph1 and podocin becomes disrupted leading to loss of slit diaphragm integrity and impaired renal function.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

See full target information NPHS1

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文献 (90)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes research 2025:2379872 PubMed41069927

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Transcriptomics and Proteomics Reveal That TLPW Acupuncture Ameliorates Proteinuria in Diabetic Kidney Disease Model Rats by Suppressing Epithelial-to-Mesenchymal Transition via the DPP4/SDF-1/TGF-/Smad Signalling Axis.

Applications

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Species

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Yue Ji,Jiya Sun,Zihao Zhuang,Yunming Xiao,Shipian Li,Zhilong Zhang,Xu Wang,Xinju Li

Frontiers in pharmacology 16:1586333 PubMed41020010

2025

Exploration of the renoprotective effect of Yi-Shen-Hua-Shi granules on db/db mice and the mechanism of podocyte apoptosis based on the GRP78/CHOP signaling pathway.

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Unspecified application

Species

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Yanmo Cai,Yunhua Liu,Sitong Wang,Ge Jin,Kaidong Zhou,Xin Zhou,Xinxue Zhang,Min Zhang,Zongjiang Zhao

Nature communications 16:6960 PubMed40730843

2025

Single urinary extracellular vesicle proteomics identifies complement receptor CD35 as a biomarker for sepsis-associated acute kidney injury.

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Ning Li,Tao-Tao Tang,Menglei Gu,Yu-Qi Fu,Wei-Wei Qian,Nan Nan Ma,An-Ran Shen,Tingting Zhu,Run-Bo Huo,Tao Zhang,Jian-Feng Xie,Lu Zhang,Bi-Cheng Liu,Lin-Li Lv

Journal of cellular physiology 240:e70053 PubMed40495439

2025

Targeting Heparanase Attenuates Podocyte Injury Induced by Puromycin Aminonucleoside.

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Species

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Xing-Yun Huang,Yu-Hsien Lu,Hsiao-Hui Lee

Archives of medical science : AMS 21:538-554 PubMed40395889

2025

Celastrol attenuates Alzheimer's disease-mediated learning and memory impairment by inhibiting endoplasmic reticulum stress-induced inflammation and oxidative stress.

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Species

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Fanfan Cao,Limin Xu,Xiaoxue He,Yongbin Chi,Ying Wang,Qiuyun Liu,Denghai Zhang

Cell death & disease 16:185 PubMed40108127

2025

NAT10 mediates TLR2 to promote podocyte senescence in adriamycin-induced nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyang Hu,Linxiao Lv,Yuqi Lei,Min Chen,Sijie Zhou,Zhangsuo Liu

Inflammation 48:3180-3193 PubMed40067577

2025

Canagliflozin Attenuates Podocyte Inflammatory Injury through Suppressing the TXNIP/NLRP3 Signaling Pathway in Diabetic Kidney Disease Mice.

Applications

Unspecified application

Species

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Siyu Li,Jie Wang,Ying Chen,Yanlu Cheng,Yanan Wang,Nuowen Xu,Hao Wang,Li Wang,Yangfeng Chi,Xiaoxue Ye,Yanting Shi,Ji Fang,Xingmei Yao,Jiebo Huang,Qing Xia,Tianli Bai,Bingbing Zhu

Renal failure 47:2459896 PubMed39972601

2025

Modified Huangqi Chifeng decoction alleviates podocyte injury on rat with experimental membranous nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Meiying Chang,Xiujie Shi,Bin Yang,Peng Li,Yifan Zhang,Qi Zhang,Yu Zhang

Nature communications 16:167 PubMed39747090

2025

Small molecule APOL1 inhibitors as a precision medicine approach for APOL1-mediated kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon Zimmerman,Leslie A Dakin,Anne Fortier,Evanthia Nanou,Angelo Blasio,James Mann,Howard Miller,Marissa Fletcher,Tiansheng Wang,Suganthini Nanthakumar,Gizelle McCarthy,Caline Matar,Prachi Matsye,Guanyu Wang,Phillip Snyder,Kevin Daniel,Harsha Swamy,Kelly Sullivan,Franklin Bright,Audrey Powers,Kevin J Gagnon,Fan Lu,Steven Paula,Suvarna Khare-Pandit,Larry Henry,Martine Hamel,Francois Denis,Olivier Nicolas,Niresh Hariparsad,Shyamesh Kumar,Jennifer Proctor,Timothy Senter,Brinley Furey,Mark E Bunnage

Open life sciences 19:20220940 PubMed39588120

2024

Renalase protects against podocyte injury by inhibiting oxidative stress and apoptosis in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yiru Wu,Yiduo Feng,Yue Yu,Yu Bai,Zongli Diao,Wenhu Liu

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