Anti-Nephrin抗体- C-terminal (ab183099)
Key features and details
- Rabbit polyclonal to Nephrin - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Nephrin抗体- C-terminal
参阅全部 Nephrin 一抗 -
描述
兔多克隆抗体to Nephrin - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human Nephrin aa 1200 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_004637)
Database link: O60500 -
阳性对照
- Mouse spleen and mouse kidney tissue lysates; Human liver cancer tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
(without Mg2+ and Ca2+) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183099于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/25 - 1/100.
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WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 134 kDa.
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说明 |
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IHC-P
1/25 - 1/100. |
WB
1/500 - 1/2000. Predicted molecular weight: 134 kDa. |
靶标
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功能
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. -
组织特异性
Specifically expressed in podocytes of kidney glomeruli. -
疾病相关
Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. -
序列相似性
Belongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains. -
发展阶段
In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli. -
翻译后修饰
Phosphorylated on tyrosine residues. -
细胞定位
Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4868 Human
- Entrez Gene: 54631 Mouse
- Entrez Gene: 64563 Rat
- Omim: 602716 Human
- SwissProt: O60500 Human
- SwissProt: Q9QZS7 Mouse
- SwissProt: Q9R044 Rat
- Unigene: 122186 Human
see all -
别名
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
see all
图片
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All lanes : Anti-Nephrin antibody - C-terminal (ab183099) at 1/800 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/5000 dilution
Predicted band size: 134 kDa
Exposure time: 1 minute8% SDS-PAGE
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Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling Nephrin with ab183099 at 1/50 dilution. Image on the right is treated with the synthetic peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab183099 被引用在 6 文献中.
- Murphy C et al. Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation. ALTEX 40:141-159 (2023). PubMed: 35791294
- Alomari G et al. Alleviation of diabetic nephropathy by zinc oxide nanoparticles in streptozotocin-induced type 1 diabetes in rats. IET Nanobiotechnol 15:473-483 (2021). PubMed: 34694755
- Alomari G et al. Gold nanoparticles attenuate albuminuria by inhibiting podocyte injury in a rat model of diabetic nephropathy. Drug Deliv Transl Res 10:216-226 (2020). PubMed: 31637677
- Rauch C et al. Differentiation of human iPSCs into functional podocytes. PLoS One 13:e0203869 (2018). PubMed: 30222766
- Wei MG et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med 2016:5031890 (2016). IHC-P ; Rat . PubMed: 27403197
- Al-Trad B et al. Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats. Diabetol Metab Syndr 7:97 (2015). IHC-P . PubMed: 26583047