Anti-NENF抗体(ab74474)
Key features and details
- Rabbit polyclonal to NENF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NENF抗体 -
描述
兔多克隆抗体to NENF -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide (Human) of 15 amino acids from near the C-terminus of NENF. (NP_037481).
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阳性对照
- Human kidney tissue lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74474于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 19 kDa). |
靶标
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功能
Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme. -
序列相似性
Belongs to the cytochrome b5 family. MAPR subfamily.
Contains 1 cytochrome b5 heme-binding domain. -
结构域
The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 29937 Human
- Omim: 611874 Human
- SwissProt: Q9UMX5 Human
- Unigene: 461787 Human
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别名
- Cell growth inhibiting protein 47 antibody
- Cell immortalization-related protein 2 antibody
- CIR2 antibody
see all
图片
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Western blot - Anti-NENF antibody (ab74474)Lane 1 : Anti-NENF antibody (ab74474) at 1 µg/ml
Lane 2 : Anti-NENF antibody (ab74474) at 2 µg/ml
All lanes : Human kidney tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 19 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab74474 被引用在 2 文献中.
- Tejedor G et al. MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis. Front Cell Dev Biol 9:579951 (2021). PubMed: 33738280
- Stefanska B et al. Genome-wide study of hypomethylated and induced genes in patients with liver cancer unravels novel anticancer targets. Clin Cancer Res 20:3118-32 (2014). WB ; Human . PubMed: 24763612