重组Anti-NELFe抗体[EPR11600] (ab170104)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11600] to NELFe
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-NELFe抗体[EPR11600]
参阅全部 NELFe 一抗 -
描述
兔单克隆抗体[EPR11600] to NELFe -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human NELFe.
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阳性对照
- WB: HeLa, T47-D, HepG2 or 293T lysate, HepG2 cells. Mouse and Rat brain lysates. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11600 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170104于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/300.
|
|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 44 kDa.
|
ICC/IF |
1/500.
For unpurified use at 1/100 - 1/250. |
|
IP |
1/10 - 1/100.
|
说明 |
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Flow Cyt (Intra)
1/300. |
WB
1/1000 - 1/5000. Predicted molecular weight: 44 kDa. |
ICC/IF
1/500. For unpurified use at 1/100 - 1/250. |
IP
1/10 - 1/100. |
靶标
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功能
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. -
组织特异性
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas. -
序列相似性
Belongs to the RRM NELF-E family.
Contains 1 RRM (RNA recognition motif) domain. -
结构域
The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation. -
翻译后修饰
Sumoylated. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7936 Human
- Entrez Gene: 27632 Mouse
- Entrez Gene: 294258 Rat
- Omim: 154040 Human
- SwissProt: P18615 Human
- SwissProt: P19426 Mouse
- Unigene: 423935 Human
- Unigene: 279907 Mouse
see all -
别名
- Major histocompatibility complex gene RD antibody
- Negative elongation factor E antibody
- Negative elongation factor polypeptide E antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NELFe with Purified ab170104 at 1:500 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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ab170104 (purified) at 1:20 dilution (2µg) immunoprecipitating NELFe in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab170104 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab170104 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NELFe with Purified ab170104 at 1/300 dilution (1µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-NELFe antibody [EPR11600] (ab170104) at 1/5000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa -
All lanes : Anti-NELFe antibody [EPR11600] (ab170104) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : T47-D cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit
at 1/2000 dilution
Predicted band size: 44 kDaThis image was generated using the unpurified version of the product.
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Immunofluorescence analysis of HepG2 cells labeling NELFe with ab170104 at a 1/100 dilution.
This image was generated using the unpurified version of the product.
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Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) or 1X PBS (negative control) (lane 2) using ab170104, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
This image was generated using the unpurified version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab170104 被引用在 12 文献中.
- Gorbovytska V et al. Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF. Nat Commun 13:2429 (2022). PubMed: 35508485
- Abe K et al. Distinct patterns of RNA polymerase II and transcriptional elongation characterize mammalian genome activation. Cell Rep 41:111865 (2022). PubMed: 36577375
- Ghouraba MH et al. Role of NELF-B in supporting epithelial-mesenchymal transition and cell proliferation during hepatocellular carcinoma progression. Oncol Lett 22:761 (2021). PubMed: 34539865
- Takahashi H et al. The role of Mediator and Little Elongation Complex in transcription termination. Nat Commun 11:1063 (2020). PubMed: 32102997
- Aoi Y et al. NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-Release. Mol Cell 78:261-274.e5 (2020). PubMed: 32155413