Anti-NEK9 (phospho T210)抗体(ab63553)
Key features and details
- Rabbit polyclonal to NEK9 (phospho T210)
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NEK9 (phospho T210)抗体
参阅全部 NEK9 一抗 -
描述
兔多克隆抗体to NEK9 (phospho T210) -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human NEK9 (phospho T210).
Database link: Q8TD19 -
阳性对照
- HeLa cells and extracts from HepG2 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63553于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 107 kDa).
|
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| ICC/IF |
1/500 - 1/1000.
|
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| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 107 kDa). |
|
ICC/IF
1/500 - 1/1000. |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. -
组织特异性
Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts. -
序列相似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Contains 6 RCC1 repeats. -
发展阶段
Expression varied mildly across the cell cycle, with highest expression observed in G1 and stationary-phase cells. -
结构域
Dimerizes through its coiled-coil domain. -
翻译后修饰
Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 91754 Human
- Entrez Gene: 217718 Mouse
- Omim: 609798 Human
- SwissProt: Q8TD19 Human
- SwissProt: Q8K1R7 Mouse
- Unigene: 7200 Human
- Unigene: 29071 Mouse
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别名
- KIAA1995 antibody
- APUG antibody
- DKFZp434D0935 antibody
see all
图片
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Western blot - Anti-NEK9 (phospho T210) antibody (ab63553)All lanes : Anti-NEK9 (phospho T210) antibody (ab63553) at 1/500 dilution
Lane 1 : extracts from HepG2 cells at 30 µg/ml
Lane 2 : extracts from HepG2 cells at 30 µg with immunizing peptide at 10 µg
Predicted band size: 107 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-NEK9 (phospho T210) antibody (ab63553)ab63553 at 1/500 dilution staining HeLa cells, without (left) and with (right) immunizing peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK9 (phospho T210) antibody (ab63553)Ab63553 staining Human colon. Staining is localised to the cytoplasm and nucleus.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab63553 被引用在 2 文献中.
- Kim M et al. Overexpression of the NEK9-EG5 axis is a novel metastatic marker in pathologic stage T3 colon cancer. Sci Rep 13:342 (2023). PubMed: 36611072
- Levinsohn JL et al. Somatic Mutations in NEK9 Cause Nevus Comedonicus. Am J Hum Genet 98:1030-1037 (2016). PubMed: 27153399