重组Anti-NEK6抗体[EPR5282] (ab109177)
![Western blot - Anti-NEK6 antibody [EPR5282] (ab109177)](https://www.abcam.cn/ps/products/109/ab109177/Images/ab109177-389439-anti-nek6-antibody-epr5282-western-blot-human-knockout-lysate.jpg "Western blot - Anti-NEK6 antibody [EPR5282] (ab109177)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5282] to NEK6
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-NEK6抗体[EPR5282]
参阅全部 NEK6 一抗 -
描述
兔单克隆抗体[EPR5282] to NEK6 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2, HeLa, 293T and HT-1080 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5282 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109177于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
靶标
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功能
Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis. Phosphorylates KIF11 to promote mitotic spindle formation. -
组织特异性
Ubiquitous, with highest expression in heart and skeletal muscle. -
序列相似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Clone ID: BC004174 Human
- Entrez Gene: 10783 Human
- Entrez Gene: 360161 Rat
- IMAGE Id: 2958695 Human
- Omim: 604884 Human
- SwissProt: Q9HC98 Human
- SwissProt: P59895 Rat
- Unigene: 197071 Human
see all -
别名
- Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
- NEK6 antibody
- NEK6_HUMAN antibody
see all
图片
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Western blot - Anti-NEK6 antibody [EPR5282] (ab109177)All lanes : Anti-NEK6 antibody [EPR5282] (ab109177) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NEK6 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDaLanes 1-3: Merged signal (red and green). Green - ab109177 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.
ab109177 Anti-NEK6 antibody [EPR5282] was shown to specifically react with NEK6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265910 (knockout cell lysate ab258072) was used. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. ab109177 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NEK6 antibody [EPR5282] (ab109177)](https://www.abcam.cn/ps/products/109/ab109177/Images/ab109177-121744-anti-nek6-antibody-epr5282-western-blot.jpg)
Western blot - Anti-NEK6 antibody [EPR5282] (ab109177)All lanes : Anti-NEK6 antibody [EPR5282] (ab109177) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDa -
![Anti-NEK6 antibody [EPR5282] (ab109177)](https://www.abcam.cn/ps/products/109/ab109177/Images/ab109177-3-benefits-of-recombinant-antibodies.png)
Anti-NEK6 antibody [EPR5282] (ab109177)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109177 被引用在 1 文献中.
- De Donato M et al. Identification and antitumor activity of a novel inhibitor of the NIMA-related kinase NEK6. Sci Rep 8:16047 (2018). PubMed: 30375481