Anti-NEK2抗体(ab115731)
Key features and details
- Rabbit polyclonal to NEK2
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-NEK2抗体
参阅全部 NEK2 一抗 -
描述
兔多克隆抗体to NEK2 -
宿主
Rabbit -
经测试应用
适用于: ELISA, ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
GRQLGEPEKSQDS
conjugated to KLH, corresponding to amino acids 287-299 of Human NEK2 -
阳性对照
- Human testis tissue; Human colon tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115731于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
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| IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not. -
组织特异性
Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. -
序列相似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
发展阶段
Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Isoform 1 and isoform 2 expression is low in the G1 phase and increases in the S/G2 phases. Isoform 1 is absent from cells arrested in the G2/M prometaphase. Isoform 2 remains present in cells arrested in the G2/M prometaphase. -
翻译后修饰
It is unsure whether Thr-170 or Ser-171 is phosphorylated. -
细胞定位
Cytoplasm. Predominantly cytoplasmic; Nucleus > nucleolus. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end and Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Co-localizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. - Information by UniProt
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数据库链接
- Entrez Gene: 4751 Human
- Omim: 604043 Human
- SwissProt: P51955 Human
- Unigene: 153704 Human
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别名
- HSPK 21 antibody
- HsPK21 antibody
- NEK 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK2 antibody (ab115731)ab115731 at 2.5ug/ml staining NEK2 in Human testis tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK2 antibody (ab115731)ab115731 at 2.5ug/ml staining NEK2 in Human colon tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab115731 被引用在 6 文献中.
- Jian F et al. NEK2 regulates cellular proliferation and cabergoline sensitivity in pituitary adenomas. J Cancer 12:2083-2091 (2021). PubMed: 33754007
- Zhou L et al. NEK2 Promotes Cell Proliferation and Glycolysis by Regulating PKM2 Abundance via Phosphorylation in Diffuse Large B-Cell Lymphoma. Front Oncol 11:677763 (2021). PubMed: 34168996
- Hu H et al. Exosome-Derived miR-486-5p Regulates Cell Cycle, Proliferation and Metastasis in Lung Adenocarcinoma via Targeting NEK2. Front Bioeng Biotechnol 8:259 (2020). PubMed: 32322578
- Chen Y et al. microRNA-128-3p overexpression inhibits breast cancer stem cell characteristics through suppression of Wnt signalling pathway by down-regulating NEK2. J Cell Mol Med 24:7353-7369 (2020). PubMed: 32558224
- Xu W et al. microRNA-138 induces cell survival and reduces WNT/ß-catenin signaling of osteoarthritis chondrocytes through NEK2. IUBMB Life 71:1355-1366 (2019). PubMed: 31034758
- Wu SM et al. Hepatoma cell functions modulated by NEK2 are associated with liver cancer progression. Int J Cancer 140:1581-1596 (2017). PubMed: 27925179