AB186519

Anti-NEK1抗体

Name: NEK1抗体 (ab186519)| Abcam中文官网
Brand: Abcam Limited
SKU: AB186519
Price: 5757 CNY
Availability: InStock

Anti-NEK1 antibody

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(1 Publication)

Rabbit Polyclonal NEK1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NEK1 aa 600-750.

查看别名

KIAA1901, NEK1, Serine/threonine-protein kinase Nek1, Never in mitosis A-related kinase 1, Renal carcinoma antigen NY-REN-55, NimA-related protein kinase 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK1 antibody (AB186519)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK1 antibody (AB186519)

Immunohistochemical analysis of paraffin-embedded Human breast tissue, labeling NEK1 using ab186519 at a 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-NEK1 antibody (AB186519)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NEK1 antibody (AB186519)

Immunofluorescence analysis of PFA/Triton X-100-fixed U-251 cells, labeling NEK1 using ab186519 at 4 μg/ml.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human NEK1 aa 600-750. The exact immunogen used to generate this antibody is proprietary information.

Q96PY6

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NEK1 also known as NIMA-related kinase 1 plays an important role in cell cycle regulation and DNA damage response. This serine/threonine-protein kinase has a molecular mass of approximately 140 kDa. NEK1 shows expression across various tissues particularly in the brain and testis. It functions by phosphorylating other proteins modifying their activity and ensuring proper cell division and stress response.

Biological function summary

NEK1 coordinates critical processes such as ciliogenesis and mitochondrial function. It participates as part of protein complexes that help maintain cellular integrity. NEK1 interacts with proteins to regulate centrosome integrity and stability ensuring proper cell polarization and division. Additionally NEK1 has a role in the DNA damage checkpoint a mechanism that maintains genomic stability.

Pathways

NEK1 integrates into the DNA damage response pathway and cilia-related signaling pathways. It interacts with key proteins such as ATR and ATM to mediate cell cycle arrest after DNA damage. NEK1 also influences the Hedgehog signaling pathway being important for ciliary function. These interactions underline its importance in cell cycle control and signaling.

NEK1 associates closely with amyotrophic lateral sclerosis (ALS) and polycystic kidney disease (PKD). Mutations in NEK1 have been linked to familial ALS indicating its role in neurodegenerative processes. In PKD NEK1 interacts with the polycystin proteins which are essential for kidney function leading to cyst formation when disrupted. These connections illustrate the impact of NEK1 dysregulation on multiple disease states.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity (PubMed : 20230784). Involved in DNA damage checkpoint control and for proper DNA damage repair (PubMed : 20230784). In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death (PubMed : 20230784). May be implicated in the control of meiosis (By similarity). Involved in cilium assembly (PubMed : 21211617).

See full target information NEK1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:19188 PubMed31844142

2019

A genome-scale CRISPR/Cas9 knockout screening reveals SH3D21 as a sensitizer for gemcitabine.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Masoudi,Motoaki Seki,Razieh Yazdanparast,Nozomu Yachie,Hiroyuki Aburatani

View all publications

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Anti-NEK1抗体

Anti-NEK1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEK1 antibody (AB186519)

Immunocytochemistry/ Immunofluorescence - Anti-NEK1 antibody (AB186519)