AB133320

重组Anti-NEAS抗体[EPR3830-43]

Anti-NEAS antibody [EPR3830-43]

RabMAb
Recombinant
了解详情

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(3 Publications)

Rabbit Recombinant Monoclonal NEAS antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

查看别名

NEAS, SPTA2, SPTAN1, Alpha-II spectrin, Fodrin alpha chain

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-NEAS antibody [EPR3830-43] (AB133320)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NEAS antibody [EPR3830-43] (AB133320)

Immunofluorescent analysis of HeLa cells labelling NEAS with ab133320 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] (AB133320)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] (AB133320)

Immunohistochemical analysis of Paraffin Embedded Human colon tissue labelling NEAS with ab133320 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] (AB133320)

Immunohistochemical analysis of Paraffin Embedded Human breast carcinoma tissue labelling NEAS with ab133320 at 1/500 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-NEAS antibody [EPR3830-43] (AB133320)

All lanes:

Western blot - Anti-NEAS antibody [EPR3830-43] (ab133320) at 1/10000 dilution

Lane 1:

HeLa cell lysate + camptothecin at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate + staurosporine at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 285 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3830-43

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

NEAS also known as Neas columbia is a protein with a molecular mass of about 45 kDa. It is expressed in various tissues including the liver heart and brain. Mechanically NEAS functions by binding to specific receptors on the cell surface and facilitates intracellular signaling processes. This protein interacts directly with receptor complexes and acts as a modulator for downstream effects making it a significant component in cellular communication and regulation.

Biological function summary

NEAS plays an important role in maintaining cellular homeostasis. It is known to be part of a larger protein complex which assists in transmitting external signals into internal cellular responses. This protein is essential for balancing cell proliferation and apoptosis contributing to normal tissue maintenance. Its activity regulates several transcription factors that influence gene expression which are vital for cellular growth and differentiation.

Pathways

NEAS is involved in the MAPK/ERK signaling and PI3K-Akt pathways. These pathways are critical for cell growth survival and metabolism. NEAS interacts with key proteins such as Raf and MEK in the MAPK/ERK pathway enhancing signal transduction for cell proliferation. It also shows an association with PDK1 a component of the PI3K-Akt pathway which plays a role in cell survival mechanisms helping cells adapt to environmental stress.

NEAS is linked to cancer and neurodegenerative diseases. Aberrant expression or malfunction of NEAS may contribute to uncontrolled cell proliferation commonly seen in cancer. In this context it associates with proteins like Bcl-2 involved in the regulation of apoptosis and could influence tumor development. For neurodegenerative diseases altered NEAS activity could impact neuronal cell survival highlighting its connection to proteins like Tau which is implicated in conditions such as Alzheimer's disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

See full target information SPTAN1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:8343-8357 PubMed40599399

2025

Combination of Ketorolac Tromethamine and Prednisolone-Loaded PLGA Nanocomposite for Effective Chronic Pain Relief in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tuyet-Nhi Do,Yen-Chin Liu,Yu-Che Chuang,Tsung-Lin Tsai,Ping-Ching Wu

Brain research bulletin 153:289-304 PubMed31539556

2019

iTRAQ-based proteomic analysis discovers potential biomarkers of diffuse axonal injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Liang,Fang Tong,Lin Zhang,Longlong Zhu,Wenhe Li,Weisheng Huang,Shuquan Zhao,Guanglong He,Yiwu Zhou

Cell cycle (Georgetown, Tex.) 18:2713-2726 PubMed31455186

2019

α-Fodrin is required for the organization of functional microtubules during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rohith Kumar Nellikka,Jamuna S Sreeja,Dhrishya Dharmapal,Rince John,Augusta Monteiro,Joana Catarina Macedo,Carlos Conde,Elsa Logarinho,Claudio E Sunkel,Suparna Sengupta

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-NEAS抗体[EPR3830-43]

Anti-NEAS antibody [EPR3830-43]

Immunocytochemistry/ Immunofluorescence - Anti-NEAS antibody [EPR3830-43] (AB133320)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] (AB133320)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEAS antibody [EPR3830-43] (AB133320)

Western blot - Anti-NEAS antibody [EPR3830-43] (AB133320)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原