Anti-NEAS抗体[3D7] (ab131575)
Key features and details
- Mouse monoclonal [3D7] to NEAS
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG1
概述
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产品名称
Anti-NEAS抗体[3D7]
参阅全部 NEAS 一抗 -
描述
小鼠单克隆抗体[3D7] to NEAS -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat -
免疫原
Recombinant fragment corresponding to Human NEAS.
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阳性对照
- Rat hippocampal lysate; cultured rat neurons and glia.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.07% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
3D7 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131575于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/3000. Detects a band of approximately 240 kDa (predicted molecular weight: 285 kDa).
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ICC/IF |
1/500.
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说明 |
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WB
1/3000. Detects a band of approximately 240 kDa (predicted molecular weight: 285 kDa). |
ICC/IF
1/500. |
靶标
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功能
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. -
疾病相关
Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia. -
序列相似性
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats. -
翻译后修饰
Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells. - Information by UniProt
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数据库链接
- Entrez Gene: 64159 Rat
- SwissProt: P16086 Rat
- Unigene: 5812 Rat
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别名
- (ALPHA)II-SPECTRIN antibody
- Alpha II Spectrin antibody
- Alpha-II spectrin antibody
see all
图片
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Anti-NEAS antibody [3D7] (ab131575) at 1/3000 dilution + Rat hippocampal lysate
Predicted band size: 285 kDa
Observed band size: 240 kDa why is the actual band size different from the predicted? -
Immunoflourescent analysis of cultured rat neurons and glia labelling NEAS with ab131575 at 1/500 dilution (green). The DNA is labelled Blue.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab131575 被引用在 1 文献中.
- Gimenez-Molina Y et al. The Differential Organization of F-Actin Alters the Distribution of Organelles in Cultured When Compared to Native Chromaffin Cells. Front Cell Neurosci 11:135 (2017). PubMed: 28522964