AB241346

Anti-NDUFS8抗体

Name: NDUFS8抗体 (ab241346)| Abcam中文官网
Brand: Abcam Limited
SKU: AB241346
Price: 5010 CNY
Availability: InStock

Anti-NDUFS8 antibody

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(2 Publications)

Rabbit Polyclonal NDUFS8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NDUFS8 aa 150 to C-terminus.

查看别名

Complex I-23kD, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit, CI-23kD, NDUFS8

2 Images

Supplier Data

Immunoprecipitation - Anti-NDUFS8 antibody (AB241346)

NDUFS8 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241346 used for IP at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab241346 at 1 μg/ml.

Lane 1 : ab241346 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-NDUFS8 antibody (ab241346)

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-NDUFS8 antibody (AB241346)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-NDUFS8 antibody (ab241346) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Predicted band size: 23 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

Synthetic Peptide within Human NDUFS8 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00217

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab241346 was affinity purified using an epitope specific to NDUFS8 immobilized on solid support.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The NDUFS8 protein also known as NADH:ubiquinone oxidoreductase core subunit S8 serves an important function in the mitochondrial electron transport chain. It weighs approximately 23 kDa and is expressed in many tissues due to its essential role in energy production. NDUFS8 is a part of the larger mitochondrial complex I where it plays a role in transferring electrons derived from NADH to ubiquinone. Through this process NDUFS8 contributes to the creation of a proton gradient used for ATP synthesis.

Biological function summary

The NDUFS8 protein functions as an integral component of the mitochondrial respiratory complex I. Complex I is essential for cellular respiration and contributes to the production of ATP which cells use as an energy source. NDUFS8 helps facilitate the electron transport chain as a subunit of this multi-protein complex ensuring effective electron transfer and energy conversion within the mitochondria.

Pathways

The NDUFS8 protein operates within the oxidative phosphorylation pathway a major energy-producing pathway in cells. It also has connections with the tricarboxylic acid (TCA) cycle where NADH is generated and enters the electron transport chain through NDUFS8. The NDUFS8 protein collaborates with other subunits of complex I such as NDUFV1 and NDUFV2 to ensure proper electron flow and energy production.

Mutations in the NDUFS8 protein can lead to mitochondrial disorders such as Leigh syndrome and mitochondrial encephalomyopathy. These conditions are characterized by severe energy production deficiencies due to impaired function of complex I. NDUFS8 mutations can interact with other proteins implicated in these disorders including NDUFAF2 which is involved in the assembly of complex I further disrupting normal mitochondrial activity and energy metabolism.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 22499348). Essential for the catalytic activity and assembly of complex I (PubMed : 22499348).

See full target information NDUFS8

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Kaohsiung journal of medical sciences 39:596-604 PubMed36912285

2023

Tan IIA mitigates vascular smooth muscle cell proliferation and migration induced by ox-LDL through the miR-137/TRPC3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Zhi Gao,Qing-Long Guan

Brain communications 4:fcac102 PubMed35602653

2022

Disruption of cellular iron homeostasis by missense variants causes severe neurodevelopmental delay, dystonia and seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Russell P Saneto,Richard Steet,Marcio A Sotero de Menezes,Cindy Skinner,Tracey A Rouault

View all publications

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Anti-NDUFS8抗体

Anti-NDUFS8 antibody

Immunoprecipitation - Anti-NDUFS8 antibody (AB241346)

Western blot - Anti-NDUFS8 antibody (AB241346)