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Anti-NDUFS3抗体[3F9DD2] (ab110246)

Reviews (6)Q&A (5)References (72)
Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  • Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  • Flow Cytometry - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)

Key features and details

  • Mouse monoclonal [3F9DD2] to NDUFS3
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 HRP

概述

  • 产品名称

    Anti-NDUFS3抗体[3F9DD2]
    参阅全部 NDUFS3 一抗

  • 描述

    小鼠单克隆抗体[3F9DD2] to NDUFS3

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Cow, Human

  • 免疫原

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • 阳性对照

    • Isolated mitochondria from Human, Bovine Rat and Mouse hearts; Cultured Human fibroblasts; HeLa cells; Human colon and cerebellum tissues.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab110246于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF (1)
Use a concentration of 5 - 10 µg/ml.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB (4)
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa.
说明
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
Use a concentration of 5 - 10 µg/ml.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 30 kDa.

靶标

  • 功能

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

  • 序列相似性

    Belongs to the complex I 30 kDa subunit family.

  • 细胞定位

    Mitochondrion inner membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CI 30 antibody
    • CI 30KD antibody
    • CI-30kD antibody
    • Complex I 30KD antibody
    • Complex I 30kDa subunit antibody
    • COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT antibody
    • Complex I-30kD antibody
    • mitochondrial antibody
    • NADH coenzyme Q reductase antibody
    • NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibody
    • NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial antibody
    • NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 antibody
    • NADH dehydrogenase ubiquinone 30 kDa subunit antibody
    • NADH-ubiquinone oxidoreductase 30 kDa subunit antibody
    • NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibody
    • NDUFS3 antibody
    • NDUS3_HUMAN antibody
    see all

图片

  • Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246)This image is courtesy of an anonymous AbReview
    All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 1/1000 dilution

    Lanes 1-2 : Whole cell lysates from NDUFS3 KO cells.
    Lanes 3-4 : Whole cell lysates from NDUFS3 WT cells.
    Lanes 5-6 : Isolated mitochondria from NDUFS3 KO cells.
    Lanes 7-8 : Isolated mitochondria from NDUFS3 WT cells.

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse monoclonal HRP conjugate at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Additional bands at: 260 kDa (possible non-specific binding), 40 kDa (possible non-specific binding)


    Exposure time: 1 minute

    See Abreview

  • Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    Western blot - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    All lanes : Anti-NDUFS3 antibody [3F9DD2] (ab110246) at 0.5 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from Rat heart at 10 µg
    Lane 4 : Isolated mitochondria from Mouse heart at 10 µg

    Predicted band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    Immunocytochemistry/ Immunofluorescence - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    ab110246, at 10 µg/ml, staining NDUFS3 in cultured Human fibroblasts by Immunofluorescence. The cells were fixed, treated for heat-induced antigen retrieval, permeabilized and then labeled with ab110246, followed by a fluorescent goat-anti-mouse IgG.
  • Flow Cytometry - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    Flow Cytometry - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    ab110246, at 1 µg/ml, staining NDUFS3 in HeLa cells by Flow Cytometry (Blue).

    Isotype control antibody, at 1 µg/ml, staining (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    ab110246, at 1/100, staining NDUFS3 in Human colon tissue fixed with 4% PFA by Immunohistochemistry.

    ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated for 1 hour with the target. Antigen retrieval was not required.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    ab110246, at 1/1000, staining NDUFS3 in formalin fixed and paraffin embedded Human cerebellum tissue by Immunohistochemistry.

    ab110246 was labeled with a universal probe (which recognizes antibodies raised in both mouse and rabbit species) for 30 minutes and a HRP-polymer probe for 30 minutes. ab110246 was diluted and incubated with the target for 1 hour. Antigen retrieval performed by 1 min pressure cooking with 1mmol EDTA, pH8.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFS3 antibody [3F9DD2] (ab110246)
    ab110246 staining NDUFS3 in Human colon tissue sections from a normal ageing patient by Immunohistochemistry. Kindly provided by Dr. L. Greaves and D. Turnbull, Mitochondrial Research Group, Newcastle University. For more details, see Taylor et al., J. Clin. Invest. 112:1351-1360 (2003).

文献 (72)

发表研究结果有使用 ab110246？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab110246 被引用在 72 文献中.

  • Sheng H  et al. A neuron-specific Isca1 knockout rat developments multiple mitochondrial dysfunction syndromes. Animal Model Exp Med 6:155-167 (2023). PubMed: 37140997
  • Bastin J  et al. Downregulation of mitochondrial complex I induces ROS production in colorectal cancer subtypes that differently controls migration. J Transl Med 21:522 (2023). PubMed: 37533102
  • Marković A  et al. Genetic Complementation of ATP Synthase Deficiency Due to Dysfunction of TMEM70 Assembly Factor in Rat. Biomedicines 10:N/A (2022). PubMed: 35203486
  • Zhu M  et al. Interleukin-13 promotes cellular senescence through inducing mitochondrial dysfunction in IgG4-related sialadenitis. Int J Oral Sci 14:29 (2022). PubMed: 35718799
  • Anzmann AF  et al. Diverse mitochondrial abnormalities in a new cellular model of TAFFAZZIN deficiency are remediated by cardiolipin-interacting small molecules. J Biol Chem 297:101005 (2021). PubMed: 34314685
View all Publications for this product

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Immunocytochemistry/ Immunofluorescence abreview for Anti-NDUFS3 antibody [3F9DD2]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Permeabilization
Yes - 0.2% Triton-X100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 Nov 24 2014

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